As previously described (7 (link)), the rat penile samples were lysed in RIPA buffer (1xTBS, 0.1% SDS, 0.004% sodium azide, 1% Nonidet P-40, 10μL/ml PMSF, 10μL/mL protease inhibitor cocktail, 0.5% sodium deoxycholate, 10μL/mL sodium orthovanadate). Protein concentration was measured using the Coomassie Plus Protein Assay Reagent™ (Pierce Biotechnology, Rockford, IL). Equal quantities (30μg) of lysates were separated on 10% sodium dodecyl sulfate gradient polyacrylamide gels and electroblotted onto PVDF membranes (Bio-Rad, Hercules, CA, USA). Then the membranes were blocked and incubated with rabbit anti-IGFBP-3 antibody (Sc-9028) or anti-IGF-1 (Sc-9013) antibody at 1:1000 dilutions, respectively (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Chemiluminescence was detected using ECL Western blotting detection reagents (Amersham, Buckinghamshire, UK). The IGFBP-3 protein expression was visualized by densitometry using the Mivnt Image analysis system (Shanghai Institute of Optical Instruments, Shanghai, China).
Rabbit anti igfbp 3 antibody sc 9028
Manufactured by Santa Cruz Biotechnology
Sourced in United States, Canada
The Rabbit anti-IGFBP-3 antibody (Sc-9028) is a primary antibody that recognizes the IGFBP-3 (Insulin-like Growth Factor Binding Protein 3) protein. It is a useful tool for researchers studying the IGFBP-3 protein and its role in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit anti igfbp 3 antibody sc 9028
1
Rat Penile Protein Expression Analysis
2
Quantifying IGFBP-3 Protein in Rat Penile Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The rat penile samples were lysed in RIPA buffer (1×TBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 0.004% sodium azide, 10 μl/ml PMSF, 10 μl/ml protease inhibitor cocktail, 10 μl/ml sodium orthovanadate). Protein concentration was measured using the Coomassie Plus Protein Assay Reagent™ (Pierce Biotechnology, Rockford, IL). Equal quantities (30 μg) of lysates were separated on 10% sodium dodecyl sulfate gradient polyacrylamide gels and electro-blotted onto PVDF membranes (Bio-Rad, Hercules, CA). The membranes were blocked and then incubated with rabbit anti-IGFBP-3 antibody (Sc-9028) at 1:1000 dilutions (Santa Cruz Biotechnology, Santa Cruz, CA). Chemiluminescence was detected using ECL Western blotting detection reagents (Amersham, Buckinghamshire, UK). The expression of IGFBP-3 protein was visualized by densitometry using the Mivnt Image analysis system (Shanghai Institute of Optical Instruments, Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!