Pluronic solution

Manufactured by Merck Group

Sourced in United Kingdom

Pluronic solution is a block copolymer surfactant widely used as a lab equipment product. It has the core function of acting as a dispersant and stabilizer in various applications.

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Lab products found in correlation

5 protocols using pluronic solution

Nanoparticle Molecular Imprinted Polymers

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Peptide sequences employed in nanoMIP development
(Ontores Biotechnology, Zhejiang, China), monomers for nanoMIP synthesis
(Sigma, Gillingham, UK), sodium hydroxide (Sigma), ammonium persulfate
(Sigma), mercaptoundecanoic acid (Sigma), ethanolamine hydrochloride
(Sigma), bovine serum albumin (BSA, Sigma), Pluronic solution (Sigma),
4-ABA (Fisher Scientific, Loughborough, UK), EDC (Fisher Scientific),
NHS (Fisher Scientific), ferricyanide (Sigma), ferrocyanide (Sigma),
potassium chloride (KCl, Sigma), hydrogen peroxide (30%, Sigma), ammonia
solution (25%, VWR International, Leicestershire, UK), PBS tablets
(Sigma), the alpha variant of the SARS-CoV-2 spike protein (The Native
Antigen Company, Kidlington, UK), the delta variant of the spike protein
(Abbexa, Cambridge, UK), HSA (Sigma), and IL-6 (Bio-Rad, Watford,
UK) were used as received. The SARS-CoV-2 RBD and ORF8 were provided
by the Medical Research Council Protein Phosphorylation and Ubiquitylation
Unit (Dundee, UK). PBS solutions were prepared with deionized (DI)
water, and AFM experiments were performed using Milli-Q water (both
with a resistivity of ≥18.2 MΩ cm).

McClements J., Bar L., Singla P., Canfarotta F., Thomson A., Czulak J., Johnson R.E., Crapnell R.D., Banks C.E., Payne B., Seyedin S., Losada-Pérez P, & Peeters M. (2022). Molecularly Imprinted Polymer Nanoparticles Enable Rapid, Reliable, and Robust Point-of-Care Thermal Detection of SARS-CoV-2. ACS Sensors, 7(4), 1122-1131.

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Fluorescent Bead Suspension for Experiments

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Fluorescent polystyrene beads of 6 ± 0.9 μm (Product No. 36-2, 15%CV, Thermo Fisher) and 18 ± 0.7 μm (Product No. 19096, 4%CV, Bangs Lab) mean diameters were used in all experiments. The 6 μm dry stock beads and the aqueous 18 μm beads were suspended and diluted, respectively, in 0.2% pluronic solution (Sigma-Aldrich) in phosphate buffer solution (PBS) to prevent particle aggregation. The weight ratio of beads in the suspension was 0.05%. The final concentration of beads was 6.3×10⁴ and 3.8×10⁴ beads/mL for the 6 μm and 18 μm beads, respectively.

Yang D., Leong S., Lei A, & Sohn L.L. (2015). High-Throughput Microfluidic Device for Rare Cell Isolation. Proceedings of SPIE--the International Society for Optical Engineering, 9518, 95180E.

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Physicochemical Characterization of MWCNTs

Cited 3 times

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Tangled (t)MWCNTs were obtained from Helix Materials Solutions Inc. (Richardson, TX). Rigid (r)MWCNTs, also known as Mitsui-7, were manufactured by Mitsui & Co. (Tokyo, Japan) but were obtained from NIOSH. Various physicochemical characteristics of tMWCNTs and rMWCNTs including rigidity, diameter, length, surface area, zeta potential, and residual trace metal content have been previously determined by our lab and others (Duke et al., 2017 (link); Hilton et al., 2017 (link); Porter et al., 2010 (link); Ryman-Rasmussen et al., 2009b (link)). These physicochemical characteristics are summarized in Supplemental Table 1. MWCNTs were suspended in 0.1% pluronic solution (Sigma, St. Louis, MO) diluted in Dulbecco’s phosphate-buffered saline (DPBS) to a concentration of 0.1 μg/ml.

Sridharan S., Taylor-Just A, & Bonner J.C. (2021). Osteopontin mRNA Expression by Rat Mesothelial Cells Exposed to Multi-Walled Carbon Nanotubes as a Potential Biomarker of Chronic Neoplastic Transformation In vitro. Toxicology in vitro : an international journal published in association with BIBRA, 73, 105126.

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Oxygen Sensing in Organoid Cultures

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Self-adhesive sensor dots (PreSens Precision Sensing GmbH) were autoclaved (121°C, 15 min) and batch calibrated using a two-point calibration in oxygen-free water and air-saturated water, according to the manufacturer’s guidelines. The oxygen-free standard was made by dissolving Na₂SO₃ (1 g) and Co(NO₃)₂ standard solution (50 µL) (ρ(Co) = 1000 mg/L; in nitric acid 0.5 mol/L) in water (100 mL). Air-saturated water was obtained by blowing air into a stirred water-filled beaker for 20 min under agitation. We observed no significant changes in the signal acquisition of non- vs autoclaved sensor dots, suggesting that sterilization did not affect the sensing capability. Next, sensor dots were glued onto 24-well plate wells pre-coated with 1% Pluronic solution in PBS (Sigma-Aldrich) for 2 h at 37°C, and seeded with organoids embedded in a drop of Matrigel or suspended in cell culture medium. Plates were incubated at 37°C for 7 days at normoxic (21% O₂) and hypoxic (5% O₂) conditions. Cell culture medium was refreshed after 4 days of culture. The concentration of dissolved oxygen was measured every 5 min from the bottom side of the 24-well plate by using a fluorescence transmitter (Oxy-SMA, PreSens Precision Sensing GmbH) connected to polymeric optic fibers and processed by using the PreSens Measurement Studio 2 software.

Kakni P., Jutten B., Teixeira Oliveira Carvalho D., Penders J., Truckenmüller R., Habibovic P, & Giselbrecht S. (2023). Hypoxia-tolerant apical-out intestinal organoids to model host-microbiome interactions. Journal of Tissue Engineering, 14, 20417314221149208.

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Micropellet Culture for Chondrogenesis

Cited 1 time

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To eliminate air bubbles retained in microwells, 3 mL of cell-free chondrogenic medium was added to each well, and plates were centrifuged for 5 min at 2000 x g (Fig. 1A). Each well was seeded with 1.2×10⁶ BMSC in 1 mL of chondrogenic medium, yielding approximately 5000 cells per microwell. Classic pellet cultures were used in validation studies, and to delineate these from micropellets (5×10³ BMSC) these cultures are referred to as “macropellets (2×10⁵ BMSC)”. These macropellet cultures were generated by seeding 2×10⁵ BMSC in 1 mL of induction medium in 96-deep well V-bottom plates (Corning). V-bottom plates were also coated with 5% Pluronic solution (Sigma-Aldrich) and rinsed well with PBS prior to cell seeding. Both plate types were centrifuged for 5 min at 150 x g to aggregate BMSC at the bottom of wells^{8 (link),11 (link)}. Cultures were maintained at 2% O₂ plus 5% CO₂ in a 37 °C incubator. Medium was exchanged every other day. A portion of the exchanged medium was collected and stored at −30 °C for future glycosaminoglycan (GAG) quantification. After the experimental period, the mesh was peeled from the PDMS discs to enable harvest of micropellets.

Music E., Klein T.J., Lott W.B, & Doran M.R. (2020). Transforming growth factor-beta stimulates human bone marrow-derived mesenchymal stem/stromal cell chondrogenesis more so than kartogenin. Scientific Reports, 10, 8340.

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