Sytox green s7020

Manufactured by Thermo Fisher Scientific

Sourced in Spain

Sytox Green (S7020) is a nucleic acid stain that can be used to detect dead cells in a population. It is a high-affinity, green-fluorescent nucleic acid stain that penetrates cells with compromised plasma membranes. The product can be used for a variety of applications including flow cytometry, fluorescence microscopy, and high-throughput screening.

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Lab products found in correlation

Ab1801, by Abcam (1 mentions) Ab18058, by Abcam (1 mentions) Ab123491, by Abcam (1 mentions) Ab2175, by Abcam (1 mentions) Colcemid, by Thermo Fisher Scientific (1 mentions) Olaparib s1060, by Selleck Chemicals (1 mentions) Propidium iodide p3566, by Thermo Fisher Scientific (1 mentions) Sc 8349, by Santa Cruz Biotechnology (1 mentions) Ab9485, by Abcam (1 mentions) Antibody against gata6, by Cell Signaling Technology (1 mentions) F4 80, by Thermo Fisher Scientific (1 mentions)

2 protocols using sytox green s7020

Detecting DNA Damage and Repair

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Mitomycin C (MMC, M0503), diepoxybutane (DEB, ref. 202533-16) 5-bromo-2-deoxyuridine (BrdU, B5002) and Hoechst 33258 (B2883) and IGEPAL (I8896) were from Sigma (Madrid, Spain). Ethidium monoazide bromide (EMA, e1374) and Sytox Green (S7020) were from Thermofischer Scientific (Barcelona, Spain). PARP inhibitor Olaparib (S1060) was from Selleckchem (Munich, Germany). Propidium iodide (P3566) and RNAse (12091021) were from Invitrogen (Barcelona, Spain). Colcemid was from Gibco (Barcelona, Spain). Antibodies used included: mouse monoclonal to CDK5RAP3 (ab57817), rabbit polyclonal to BRCA2 (ab123491), mouse monoclonal to RPA32 (ab2175), rabbit polyclonal to actin (ab1801), rabbit polyclonal to GAPDH (ab9485) and mouse monoclonal to vinculin (ab18058) were from Abcam (Cambridge, UK). Rabbit polyclonal to RAD51 (SC8349) was from Santa Cruz biotechnology (Heidelberg, Germany).

Minguillón J., Ramírez M.J., Rovirosa L., Bustamante-Madrid P., Camps-Fajol C., Ruiz de Garibay G., Shimelis H., Montanuy H., Pujol R., Hernandez G., Bogliolo M., Castillo P., Soucy P., Martrat G., Gómez A., Cuadras D., García M.J., Gayarre J., Lázaro C., Benítez J., Couch F.J., Pujana M.A, & Surrallés J. (2022). CDK5RAP3, a New BRCA2 Partner That Regulates DNA Repair, Is Associated with Breast Cancer Survival. Cancers, 14(2), 353.

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Multicolor Flow Cytometry Analysis

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Antibodies against CD11b (#17-0112-82; M1/70) (1:100 dilution), CD31 (#12-0311-82; PECAM-1, 390) (1:100 dilution), CD45 (#45-0451-82; 30-F11) (1:150 dilution), F4/80 (#12-4801-82; BM8) (1:100 dilution) were obtained from eBioscience. Antibodies against Ly6G (#127612; 1A8) (1:100 dilution) were obtained from Biolegend. Antibody against CD44 (#553134; IM7) (1:150 dilution) was obtained from BD Biosciences. Antibody against GATA6 (#26452; D61E4) (1:50 dilution) was obtained from Cell Signaling Technology. SYTOX Green (S7020) was obtained from Thermo Fisher Scientific. For intravital imaging, we used a very low concentration at 2 μg per mouse of each antibody. Each antibody was diluted in PBS as appropriate.

Honda M., Kadohisa M., Yoshii D., Komohara Y, & Hibi T. (2021). Directly recruited GATA6 + peritoneal cavity macrophages contribute to the repair of intestinal serosal injury. Nature Communications, 12, 7294.

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