Cells (5000 cells per well) were seeded in 8-well chamber slides (Lab-Tek) and incubated overnight. Cells were then exposed to F-AuNSs (100 μg per mL) at different time points (2, 4, 6, 8, 12, 18, and 24 h), fixed with 4% formaldehyde solution for 10 min, permeabilized with 0.1% Triton X-100 for 1 h, and blocked with 10% normal goat serum solution at room temperature for 1 h. Cells were incubated overnight at 4 °C with anti-EEA1 antibody (Abcam, ab109110, 1/250) for early endosome staining or anti-RAB7 antibody (Abcam, ab126712, 1/50) for late endosome staining, and washed three times followed by staining with Alexa Fluor 594-conjugated anti-rabbit IgG (Abcam, ab150080, 1/500). For lysosome imaging, cells were stained with BioTracker NIR 633 (Sigma, 1/500) according to the manufacturer’s instructions. Slides were mounted with DAPI nuclear dye and visualized under a Leica SP5 X MP confocal microscope. Images were captured utilizing a 63× objective.
Biotracker nir 633
Manufactured by Merck Group
BioTracker NIR 633 is a near-infrared spectroscopy (NIRS) instrument designed for laboratory use. It measures the absorption and reflection of near-infrared light to provide information about the chemical composition and structure of samples. The device operates using a 633 nm laser source.
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Lab products found in correlation
2 protocols using biotracker nir 633
1
Intracellular Trafficking of F-AuNSs
2
Intracellular Trafficking of F-AuNSs
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Cells (5000 cells per well) were seeded in 8-well chamber slides (Lab-Tek) and incubated overnight. Cells were then exposed to F-AuNSs (100 μg per mL) at different time points (2, 4, 6, 8, 12, 18, and 24 h), fixed with 4% formaldehyde solution for 10 min, permeabilized with 0.1% Triton X-100 for 1 h, and blocked with 10% normal goat serum solution at room temperature for 1 h. Cells were incubated overnight at 4 °C with anti-EEA1 antibody (Abcam, ab109110, 1/250) for early endosome staining or anti-RAB7 antibody (Abcam, ab126712, 1/50) for late endosome staining, and washed three times followed by staining with Alexa Fluor 594-conjugated anti-rabbit IgG (Abcam, ab150080, 1/500). For lysosome imaging, cells were stained with BioTracker NIR 633 (Sigma, 1/500) according to the manufacturer’s instructions. Slides were mounted with DAPI nuclear dye and visualized under a Leica SP5 X MP confocal microscope. Images were captured utilizing a 63× objective.
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