The circulating plasma levels of glucose (41011, Spinreact, Sant Esteve d’en Bas, Spain), free fatty acids (FFA) (MAK044, Sigma-Aldrich, Tres Cantos, Spain), high-density lipoproteins (HDL) and low-/very-low density lipoproteins (LDL/VLDL) (MAK045, Sigma-Aldrich, Tres Cantos, Spain), as well as TG (41030, Spinreact, Sant Esteve d’en Bas, Spain), were measured at week 8. The metabolites were determined by enzymatic colorimetric methods following the manufacturers’ instructions (n = 10).
Mak045
Manufactured by Merck Group
Sourced in Germany
The MAK045 is a laboratory equipment product manufactured by the Merck Group. It is designed for general laboratory use, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. More information would be required to present a concise and accurate description without interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Mak266, by Merck Group (3 mentions)
Mak043, by Merck Group (2 mentions)
Mak044, by Merck Group (1 mentions)
Mak187, by Merck Group (1 mentions)
Infinite f200 pro, by Tecan (1 mentions)
Adiponectin, by R&D Systems (1 mentions)
Leptin, by BioVendor (1 mentions)
Colorimetric assay kit, by Merck Group (1 mentions)
Mak175, by Merck Group (1 mentions)
7 protocols using mak045
1
Metabolic Profile Measurements
2
Serum Lipid and Antioxidant Biomarkers
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Serum total cholesterol, triglyceride, and low-density lipoprotein-cholesterol (LDL-C) concentrations were determined colorimetrically with a spectrophotometer using triglyceride (TR0100), total cholesterol (MAK043), and LDL/VLDL (MAK045) kits from Sigma Aldrich, following the manufacturer's instructions. Serum and meat filtrates were used for measuring the activity of the glutathione peroxidase (GSH-Px) using a commercial assay kit (Sigma-Aldrich, G6137). Total antioxidant capacity (T-AOC) was determined using a commercial assay kit (Sigma-Aldrich, MAK187), and the activity of superoxide dismutase (SOD) enzyme was determined using a commercial assay kit (Sigma-Aldrich, 19160) following the instructions of the test kit.
3
Plasma Lipid and Protein Profiling
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Concentration of plasma lipids was measured using commercially available reagents, according to manufacturer’s instructions (HDL and LDL, Sigma-Aldrich, #MAK045; Triglycerides, Sigma-Aldrich, #MAK266).
Sandwich ELISA was performed on plasma samples for the quantitative analysis of target proteins using commercially available kits, according to manufacturer’s instructions. Adiponectin: R&D Systems, Minneapolis, MN, USA, #RRP300; Leptin: BioVendor, Brno, Czech Republic, #RD291001200R; IGF-1: Medignost, Reutlingen, Germany, #E25; BDNF Merck, Darmstadt, Germany.
Sandwich ELISA was also performed on total protein extracts from the hippocampus, for the analysis of BDNF, using kit #RAB1138 (Sigma-Aldrich).
Final absorbance was read on a microplate reader (Infinite F200 pro, Tecan, Männedorf, Switzerland).
Sandwich ELISA was performed on plasma samples for the quantitative analysis of target proteins using commercially available kits, according to manufacturer’s instructions. Adiponectin: R&D Systems, Minneapolis, MN, USA, #RRP300; Leptin: BioVendor, Brno, Czech Republic, #RD291001200R; IGF-1: Medignost, Reutlingen, Germany, #E25; BDNF Merck, Darmstadt, Germany.
Sandwich ELISA was also performed on total protein extracts from the hippocampus, for the analysis of BDNF, using kit #RAB1138 (Sigma-Aldrich).
Final absorbance was read on a microplate reader (Infinite F200 pro, Tecan, Männedorf, Switzerland).
4
Serum Lipid Measurement Protocol
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Total serum cholesterol-, LDL-C/VLDL-C, HDL-C and triglyceride concentrations were measured by a commercially available assay in accordance with instructions provided by the manufacturer (MAK043, MAK045 and MAK266, Sigma Aldrich). Assay readings were measured using a FLUOstar® Omega multi-mode plate reader using spectrophotometer (BMG Labtech, Ortenberg, Germany).
5
Quantification of Lipid and Glucose Levels
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Commercially available colorimetric assay kits manufactured by Sigma-Aldrich, Germany, were used for the serum levels of Total Cholesterol (Catalog Number CS0005; mg/dl; Detection range: 1–5 μg), Triglycerides (Catalog Number MAK266; mg/dl; Sensitivity: 2 pmole–10 nmole; Detection range: 2–10,000 μM range), HDL-Cholesterol (Catalog Number MAK045; mg/dl; Detection range: 1–5 μg). The manuals provided in the kits were followed for the assay procedure of serum total cholesterol, serum triglycerides, and serum HDL-cholesterol level determination. Serum LDL-Cholesterol was calculated by using the Friedrick equation. Serum glucose was quantified through Bioclin® Glucose Monoreagent diagnostic kit having detection range of 2-500 mg/dl with CV% < 3.11.
6
Plasma Lipid Extraction and Quantification
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Extraction of plasma lipids was done with the help of chloroform/methanol mixture (2 : 1, v/v) by following the method described by Yassin et al. [39 (link)]. Total lipid contents in plasma extracts were gravimetrically determined through solvent evaporation with the help of rotary evaporator (BIOLAND RE-5000A China). Parameters of plasma lipids like total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and triacylglycerol (TG) levels were calculated through enzymatic method, with the help of commercial kits (TR0100, MAK045, MAK175 Sigma-Aldrich, Germany). Low-density lipoprotein cholesterol (LDL-C) was quantified by the Friedewald equation (Friedewald et al., 1972):
7
Quantification of HDL-c and LDL-c
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The HDL-c and LDL-c concentrations (µg/mL) were determined using a commercial quantification kit (Sigma-Aldrich, Catalogue MAK045) as per manufacturer's protocol. Serum HDL-c and LDL-c were first separated and then the cholesterol concentration of each was determined by a coupled enzyme assay, which resulted in a colorimetric product read at 570 nm in spectrophotometer (Phoenix-2000V UV-VIS, Biotech Engineering Management Co. Ltd. (UK).
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