For the described experiments the following materials and chemicals were used: Minimal essential medium (MEM), glutamine, Hanks' Balanced Salt Solution (HBSS), normal horse serum (NHS; Gibco BRL Life Technologies, Eggenstein, Germany), glucose (Braun, Melsungen, Germany), cell-culture inserts with a pore size of 0.4 µm (Millipore, Schwalbach/Ts., Germany), vibratome (Vibratom VT 1200 S; Leica Microsystems AG, Wetzlar, Germany), six-well culture dishes (Falcon, BD Biosciences Discovery Labware, Bedford, MA, USA), streptomycin, penicillin, ascorbic acid, N-methyl-D-aspartate (NMDA; Sigma-Aldrich, Deisenhofen, Germany), insulin (Boehringer, Mannheim, Germany), ketamine hydrochloride (Merial, Hallbergmoos, Germany), xylacin (Bayer, Leverkusen, Germany, atropine (Ratiopharm, Ulm, Germany), bicinchoninic acid (BCA) test, Tris (Roth, Karlsruhe, Germany), Glycerol (Sigma-Aldrich), β-mercaptoethanol (Sigma-Aldrich, Steinheim, Germany), bromophenol blue (AppliChem, Darmstadt, Germany), chemiluminescence, superfrost microscope slides from (Thermo Fisher Scientific, Rockford, IL, USA), cryostat 3050 S (Leica), coated slides (Menzel, Braunschweig, Germany), X-ray films (Kodak, Stuttgart, Germany), Dako fluorescent mounting medium (Dako, Hamburg, Germany), Entellan (Merck, Darmstadt, Germany)
Bromophenol blue
Manufactured by AppliChem
Sourced in Germany
Bromophenol blue is a pH indicator dye used in various laboratory applications. It exhibits a blue color at pH values above 4.6 and a yellow color at pH values below 3.0. Bromophenol blue is commonly used for monitoring the progress of electrophoresis and chromatography processes.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture insert, by Merck Group (1 mentions)
Cryostat 3050, by Leica camera (1 mentions)
Insulin, by Boehringer Ingelheim (1 mentions)
Xylacin, by Bayer (1 mentions)
N methyl d aspartate nmda, by Merck Group (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
Superfrost microscope slide, by Thermo Fisher Scientific (1 mentions)
Dako fluorescent mounting medium, by Agilent Technologies (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Entellan, by Merck Group (1 mentions)
X ray film, by Kodak (1 mentions)
Glucose, by Merck Group (1 mentions)
L cysteine, by AppliChem (1 mentions)
2 protocols using bromophenol blue
1
Primary Neuron Culture and Analysis
2
Enumeration of Microbiological Diversity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stored samples were defrosted at room temperature, serially diluted in peptone water (0.1% w/v) and spread in duplicates onto agar plates of a battery of six different culture media as follows: i) Brain Heart Infusion (BHI; Biokar Diagnostics, Beauvais, France) supplemented with 0.05% L-cysteine (AppliChem, Darmstadt, Germany); ii) de Man-Rogosa-Sharpe (MRS; Biokar Diagnostics) supplemented with 0.02 g/L bromophenol blue (AppliChem) and L-cysteine (MRS-BPB; Lee and Lee, 2008); iii) Reinforced Clostridial Medium (RCM; Biokar Diagnostics); iv) Violet Red Bile with glucose Agar (VRBG; CONDA, Madrid, Spain); v) GYECN medium based on GYEC medium (Ntougias and Russell, 2001) composed of 1% (w/v) glucose (Sigma), 0.5%
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!