Bml ca1137

Manufactured by Enzo Life Sciences

BML-CA1137 is a laboratory equipment product offered by Enzo Life Sciences. It is used for specific scientific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information may be available from the manufacturer.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (3 mentions) Draq5, by Cell Signaling Technology (1 mentions) Mab377, by Merck Group (1 mentions) Af4464, by R&D Systems (1 mentions) Mab5262, by Merck Group (1 mentions) Sc 211, by Santa Cruz Biotechnology (1 mentions) Clone mec 13, by BD (1 mentions) Lsm 510 confocal microscope, by Zeiss (1 mentions) N4142, by Merck Group (1 mentions)

2 protocols using bml ca1137

Immunohistochemical Profiling of ENTPD3 in Mice

Cited 1 time

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Tissue sections from 3 WT and 3
Entpd3
^-/- mice were stained immunohistochemically as previously reported (
Taylor-Blake & Zylka, 2010 (link)). Antibodies used were: polyclonal sheep anti-mouse ENTPD3/CD39L3 (skin, 1:75; DRG and spinal cord 1:400; AF4464, R&D Systems), monoclonal mouse anti-NeuN (1:250; MAB377, Millipore), polyclonal chicken anti-Prostatic acid phosphatase (1:4,000; PAP, Aves Labs), polyclonal rabbit anti-NF200 (1:800; N4142, Sigma), monoclonal mouse anti-NF200 (Clone RT97; MAB5262, Millipore), polyclonal rabbit anti-CGRP (1:150; BML-CA1134, Enzo Life Sciences), polyclonal sheep anti-CGRP (1:300; BML-CA1137, Enzo Life Sciences); polyclonal rabbit anti-PKCγ (1:800; sc-211, Santa Cruz), and polyclonal rat anti-PECAM1/CD31 (1:400; Clone MEC 13.3, 553370, BD Biosciences). IB4 conjugated with Alexa Fluor dyes and secondary antibodies conjugated with Alexa Fluor dyes were purchased from Invitrogen. DRAQ5 (Catalog # 4084) was purchased from Cell Signaling Technology. Stained sections were imaged on a Zeiss LSM 510 confocal microscope or a Zeiss LSM 710 confocal microscope.

McCoy E., Street S., Taylor-Blake B., Yi J., Edwards M., Wightman M, & Zylka M. (2014). Deletion of ENTPD3 does not impair nucleotide hydrolysis in primary somatosensory neurons or spinal cord. F1000Research, 3, 163.

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Quantification of CGRP and NPY Neurons in DRG

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Immunohistochemistry was performed as previously described.^{23 (link)} Ten micrometer cryostat sections of upper (L1-3) and lower (L4-6) DRG were incubated with anti-CGRP (1:500, Cat#BML-CA1137, Lot#01101327; Enzo Life Sciences, Farmingdale, NY) and Guinea pig-derived anti-NPY (1:500; Cat#AB10341, Lot#GR12360; Abcam, Tokyo, Japan), and visualized with Cy3-goat anti-sheep IgG and Cy2-goat anti-guinea pig IgG (1:500; Jackson Immuno Research, West Grove, PA) with an Olympus BX-5. Ten sections each from the upper and lower DRG per mouse were randomly selected for analysis. The proportion of CGRP-immunoreactive (ir) and NPY-ir neurons among total DRG neurons was determined.

Suzuki M., Millecamps M., Ohtori S., Mori C, & Stone L.S. (2018). Anti–nerve growth factor therapy attenuates cutaneous hypersensitivity and musculoskeletal discomfort in mice with osteoporosis. Pain Reports, 3(3), e652.

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