Easytaq dna polymerase

Human ASM cells were purchased from ScienCell (Corte Del Cedro Carlsbad, CA, USA). E. coli BL21(DE3) and the pET28(a)+ vector were purchased from Novagen (Germany). The recombinant human CTGF/CCN2 C-terminal domain, Cyr61/CCN1 and NOV/CCN3 were purchased from PROSPEC (USA). The monoclonal mouse anti-human CTGF/CCN2 C-terminus, Cyr61/CCN1 and NOV/CCN3 monoclonal antibody were purchased from R&D system (USA). The anti-6xHis monoclonal antibody was from abcam (UK). The Cell_Light EdU DNA Cell Proliferation Kit was from Ribobio (China). LY294002 (PI3K inhibitor), rabbit antibodies against Akt, mTOR, β-actin, phosphorylated-Akt (Ser473), and phosphorylated-mTOR, and HRP-goat anti-rabbit IgG were purchased from Cell Signaling (USA). NcoI, XhoI, T4 DNA ligase and Easy Taq DNA polymerase were purchased from TaKaRa (Japan). The BCA protein assay kit was provided by Tiangen (China). Cell culture media were purchased from Gibco BRL (USA). The newborn calf serum was obtained from Shiqing (China). Isopropyl-1-thio-β-D-galactoside (IPTG) was purchased from Sigma-Aldrich. The His-bind resin column and total protein extraction kit were purchased from KeyGEN (China).

ω-AmDDA was purchased from TCI (Shanghai, China). DDA and ω-OHDDA were purchased from Adamas-beta Ltd. (Shanghai, China). ABTS and other chemicals were purchased from Sangon Biotech Ltd. (Shanghai, China). Easy Taq DNA polymerase, PrimeSTAR^® HS DNA Polymerase, restriction endonucleases and T4 DNA ligase were purchased from TAKARA Ltd. (Dalian, China). HRP was purchased from Sangon Biotech Ltd. (Shanghai, China). Oligonucleotides were synthesized by Sangon Biotech Ltd. (Shanghai, China) and Tsingke Biotechnology Co., Ltd. (Beijing, China).