L7 55
The L7-55 is a centrifuge designed for laboratory applications. It can accommodate sample volumes up to 4 liters and provides controlled acceleration and deceleration for precise separations. The L7-55 features a durable stainless steel chamber and a digital display for monitoring run parameters.
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10 protocols using l7 55
Isolation of Membrane Protein Fractions
Isolating Cell Membranes and Lipid Rafts
Microvesicles Isolation from Bacterial Cultures
Bacillus thuringiensis Spore Isolation
To isolate parasporal bodies, spore/parasporal body mixtures collected from 50 ml cultures were resuspended in 15 ml ddH2O and sonicated twice at 50% duty cycle for 15 s using the Ultrasonic Homogenizer 4710 (Cole-Parmer Instrument Co.). Five-milliliter samples were loaded onto a sucrose gradient cushion (30–65% w/v), which was then centrifuged at 20,000 g for 45 min at 20 °C in a Beckman L7–55 ultracentrifuge using the SW28 rotor. Bands containing parasporal bodies were collected and washed twice in ddH2O, followed by centrifugation at 6,500 g for 15 min at 4 °C after each wash and lyophilized for storage.
Purification and Characterization of Phage
Lentiviral Vectors for Cell Cycle Monitoring
Lipid Peroxidation and Antioxidant Analysis in Tadpoles
Transmission Electron Microscopy of Phage
Following the density gradient centrifugation, five microliters of the phage suspension was loaded onto 400 mesh carbon-formvar-coated copper grids and negatively stained with 1% uranyl acetate. Phage particles were visualized using a JEM-100C transmission electron microscope (JEOL, Akishima, Japan) with an accelerating voltage of 80 kV. Images were taken on Kodak film SO-163 (Kodak, Cat. #74144, Hatfield, PA, USA) with 45,000× magnification. Phage particle dimensions were measured for 10 phage particles using ImageJ version 1.53e [56 (link)] in relation to the scale bar generated from the microscope.
Plant Extract Preparation for Biochemical Analysis
Donkey Milk Fractionation Protocol
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