Mccoy s 5a medium modified atcc 30 2007

N-lauroylsarcosine sodium, 3-aminopropyltriethoxysilane (APTES), tetraethyl orthosilicate (TEOS), ammonium acetate, 5-fluorouracil, and dexamethasone were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). Carbopol 934 was purchased from Acros Organics (Morris Plains, NJ, USA). All other chemical reagents used were of analytical grade and used without further purification. HT-144 (ATCC^® HTB-63™), and McCoy’s 5a Medium Modified (ATCC^® 30-2007™) was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

The human colorectal carcinoma cell line HT-29 (ATCC^® HTB-38™), purchased from ATCC (American Type Cell Collection), was cultured in specific medium: McCoy’s 5a modified medium (ATCC^® 30-2007™) completed with 10% fetal bovine serum (FBS, Gibco) and 1% of antibiotic mixture (penicillin and streptomycin, Sigma Aldrich, Merck KGaA Darmstadt, Germany). Standard conditions were implemented for cell culturing: a temperature of 37 °C and 5% CO₂ in a humidified incubator.
Aspartame powder, penicillin and streptomycin mixture, phosphate-buffered saline (PBS), trypsin EDTA, Trypan blue, and Alamar blue (resazurin) were acquired from Sigma Aldrich, Merck KGaA. The cell culture specific media—McCoy’s 5a Medium Modified (ATCC^® 30-2007™) and fetal bovine serum (FBS, Gibco)—were purchased from ATCC and Thermo Fisher Scientific, Inc., Waltham, MA, USA, respectively. All the reagents were of analytical standard purity and were applied according to the manufacturers’ recommendations.