Micellar lipid solubility in the presence of casein digest, EPL digest, and l -lysine was measured by the method of Nagaoka et al. (4 (link)) with some modifications. The micellar solutions were incubated at 37°C for 24 h and then 0.5 mL of digest products and l -lysine (0.05 g/mL) were individually mixed with 0.5 mL of micellar solutions. Micellar solutions (0.5 mL) containing 6.6 mmol/L sodium taurocholate, 1.5 mmol/L cholesterol, 1 mmol/L oleic acid, 0.5 mmol/L monoolein, 0.6 mmol/L phosphatidylcholine, 132 mmol/L NaCl, and 15 mmol/L sodium phosphate (pH 7.4) were treated by sonication. The mixture was incubated at 37°C for 1 h, and then ultracentrifuged at 100,000 g for 1 h at 37°C. The supernatant was collected for the determination of cholesterol, fatty acid, phospholipid, and bile acid concentrations using an enzymatic assay kit (Cholesterol-E-Test Wako, Wako NEFA C-test kit, Phospholipids C-test Wako, and Total Bile Acid Test Kit; Wako Pure Chemical Industries Ltd.).
Total bile acid test kit
Manufactured by Fujifilm
Sourced in Japan
The Total Bile Acid Test Kit is a laboratory equipment product designed to quantitatively measure the total bile acid concentration in biological samples. It provides an analytical tool for clinicians and researchers to assess bile acid levels, which can be indicative of various liver and gastrointestinal conditions.
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Lab products found in correlation
Phospholipids c test wako, by Fujifilm (1 mentions)
Wako nefa c test kit, by Fujifilm (1 mentions)
Cholesterol e test wako, by Fujifilm (1 mentions)
Varioskan plate reader, by Thermo Fisher Scientific (1 mentions)
Au5431 automatic analyzer, by Olympus (1 mentions)
Triglyceride e test, by Fujifilm (1 mentions)
4 protocols using total bile acid test kit
1
Micellar Lipid Solubility Modulated by Protein Digests
2
Fecal Total Bile Acid Quantification
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A total bile acid-test kit (Wako, Osaka, Japan) was utilized to measure fecal total bile acids (TBA) according to the manufacturer’s instruction. First, fecal lipid extraction was executed as described above. Using the enzymatic calorimetric method, the reacted substrates create end color products that are directly proportional to fecal TBA contents. Absorbance was measured at a wavelength of 560 nm by a Varioskan plate reader (Thermo Scientific, Waltham, MA, USA) and expressed as fold change with respect to the HC group.
3
Bile Acid Binding Capacity of Protein Digests
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Bile acid binding capacities of the casein digest, EPL digest, and l -lysine were measured by the method of Takahashi et al. (11 (link)). The bile acid content was measured using an enzymatic assay kit (Total Bile Acid Test Kit; Wako Pure Chemical Industries Ltd., Osaka, Japan).
4
Lipid Profiling in Mice Model
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The serum TAG, PL, total cholesterol, high‐density lipoprotein cholesterol (HDL‐C), and non‐high‐density lipoprotein cholesterol (non‐HDL‐C) contents were measured with an Olympus AU5431 automatic analyzer (Olympus Co.) at Japan Medical Laboratory.
The liver total lipid was extracted using the method of Bligh and Dyer (1959 ). The liver TAG and PL contents were measured by Triglyceride E Test (Fujifilm Wako Pure Chemical Co.) and a phosphorus assay (Rouser et al., 1970 ). The cholesterol contents in the liver and epididymal WAT were analyzed by GC as described above. The main FA contents of the total lipid were also determined by GC as described above, and utilizing tridecanoic acid (C13:0‐FA), as an internal standard.
The fecal neutral sterol, including the cholesterol and coprostanol, contents were analyzed by GC, as described above (Kaneda et al.,1980 ). The fecal total bile acid (BA) content was analyzed with the total bile acid test kit (Fujifilm Wako Pure Chemical Co.), following the manufacturer's instructions. The fecal total sterol contents were calculated by adding the neutral sterols and the total BA contents.
The liver total lipid was extracted using the method of Bligh and Dyer (
The fecal neutral sterol, including the cholesterol and coprostanol, contents were analyzed by GC, as described above (Kaneda et al.,
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