Goat anti cxcl10

Manufactured by R&D Systems

Goat anti-CXCL10 is a polyclonal antibody raised in goats against the human chemokine CXCL10. It is intended for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti goat alexafluor594, by Thermo Fisher Scientific (1 mentions) Goat anti cxcl13, by R&D Systems (1 mentions) Alexa fluor 488 anti rabbit, by Thermo Fisher Scientific (1 mentions) Confocal fluorescence microscopy, by Zeiss (1 mentions)

2 protocols using goat anti cxcl10

Immunohistochemical Analysis of Chemokines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adjacent 1-in-12 series of coronal free-floating sections (n = 5 per genotype and age) were incubated with 50 mM NH₄Cl for 30 min to reduce non-specific background staining and blocked with 15 % fetal calf serum (FCS) diluted in TBS/0.3 %Triton X-100 (TTX) for 1 h. The sections were incubated with the primary antibodies rabbit anti-ionized calcium-binding adaptor molecule 1 (IBA1; Wako) combined with goat anti-CXCL13, goat anti-CXCL10 (both R&D Systems), or rabbit anti-CXCL1 (Novus Biologicals) in 10 % FCS/TTX for 72 h at 4 °C. The secondary antibodies anti-rabbit Alexa Fluor 488 and anti-goat Alexa Fluor 594 (Invitrogen) were applied for 2 h at RT, and mounted sections were examined using a fluorescence microscope.

Okuneva O., Li Z., Körber I., Tegelberg S., Joensuu T., Tian L, & Lehesjoki A.E. (2016). Brain inflammation is accompanied by peripheral inflammation in Cstb−/− mice, a model for progressive myoclonus epilepsy. Journal of Neuroinflammation, 13, 298.

+ Open protocol

+ Expand

Visualizing CXCR3 Expression and Internalization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue sections were fixed with 5% paraformaldehyde for 10 min and frozen in OCT-embedding medium. Frozen sections (8-μm thick) were blocked with 5% normal rabbit serum for 30 min and incubated overnight with goat anti-CXCR9 or goat anti-CXCL10 (10 μg/ml; R&D) at 4°C. Slides were rinsed and incubated with rabbit anti-goat IgG Alexa Fluor 555 and DAPI for 30 min and then mounted and analyzed via confocal fluorescence microscopy (Zeiss, Germany). For ligand-induced receptor internalization, mouse effector T cells were prepared according to standard protocols (Meiser et al., 2008 (link)). Following staining with rabbit anti-mouse CXCR3 (5 μg/ml), cells were washed and incubated with or without CXCL11 (500 ng/ml) at 37°C for 30 min. Cells were then fixed in 4% paraformaldehyde and permeabilized in 1 % saponin buffer, blocked and stained with Cy3-conjugated goat anti-rabbit IgG at 4°C for 30 min. Slides were mounted and then analyzed via confocal fluorescence microscopy (Zeiss, Germany).

Wang W., Chong W.P., Li C., Chen Z., Wu S., Zhou H., Wan Y., Chen W., Gery I., Liu Y., Caspi R.R, & Chen J. (2019). Type I Interferon Therapy Limits CNS Autoimmunity by Inhibiting CXCR3-Mediated Trafficking of Pathogenic Effector T Cells. Cell reports, 28(2), 486-497.e4.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!