48 h after transfection, HCT116 and LoVo cells were seeded in to a 96‐well plate at a density of 1500 cell/well and cultured in a humidified cell culture incubator for 0, 24, 48, 72 h, respectively. Subsequently, 10 μL CCK8 reaction solution (Solarbio) was added to the cell culture at indicated time point and incubated for 1 h in a humidified cell culture incubator. The light absorption value (OD value) in each condition was captured at 450 nm wavelength on a Bio‐Rad microplate reader (Biorad).
Cck8 reaction solution
Manufactured by Solarbio
Sourced in China
The CCK8 reaction solution is a colorimetric assay kit used to measure cell viability and proliferation. It contains a tetrazolium salt that is reduced by metabolically active cells, resulting in the formation of a colored formazan product that can be quantified using a spectrophotometer.
Automatically generated - may contain errors
Lab products found in correlation
Synergy h1, by Synergy Software (2 mentions)
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Rad microplate reader, by Bio-Rad (1 mentions)
Lipofectaminetm 3000, by Thermo Fisher Scientific (1 mentions)
Synergy h1 microplate reader, by Beckman Coulter (1 mentions)
Multifunctional microplate reader, by Agilent Technologies (1 mentions)
11 protocols using cck8 reaction solution
1
Cell Viability Assay for HCT116 and LoVo Cells
2
Cell Viability Assay with Carfilzomib
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MPC-11 cells were seeded into a 96-well plate at a density of 1500 cells/well and cultured in a humidified cell culture incubator for 0, 24, 48, and 72 h in the presence of 4 nM carfilzomib. Subsequently, 10 μL of CCK-8 reaction solution (Solarbio, Beijing, China) was added to the cell culture at indicated time points and incubated for 3 h in the humidified cell culture incubator. The light absorption value (OD value) of each condition was measured at a wavelength of 450 nm using the Synergy H1 microplate reader (Winooski, Vermont, CA, USA).
3
Cell Viability Assay of OSCC Cells
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OSCC cells were seeded into 96-well plates at a density of 2 × 103 cells/well, respectively. LipofectamineTM3000 (Thermo Fisher Scientific, USA) was utilized to perform transfection as described above. After transfection, cells were further cultured for 24, 48, 72, or 96 hours. Ten microliter CCK8 reaction solution (Solarbio, CA1210, Beijing, China) was added to the cell culture at indicated time point and incubated for 1 h in a humidified cell culture incubator. The light absorption value (OD value) in each condition was captured at 450 nm wavelength on a microplate reader (Thermo Fisher Scientific).
4
Cell Viability Assessment via CCK8
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Cells of different treatments were seeded in to a 96-well plate at a density of 1500 cells/well and cultured in a humidified cell culture incubator for 48 h. 10 μL CCK8 reaction solution (Solarbio, Beijing, China) was added to the cell culture and incubated for 1 h in a humidified cell culture incubator. The light absorption value (OD value) in each condition was captured at 450 nm wavelength on a Synergy H1 microplate reader.
5
Cell Viability Assay using CCK8
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MLE-12 cells in different treatment groups were harvested by 0.25% trypsin digestion and cells were seeded in a 96-well-plate at a density of 1500 cell/well. Cells were cultured in a humidified cell culture incubator for 0, 24, 48, 72 and 96 hours, respectively. 10 μL CCK8 reaction solution (Solarbio, Beijing, China) was added to the cell culture at indicated time point and incubated for 3 h in a humidified cell culture incubator. The light absorption value (OD value) was recorded at 450 nm wavelength on a Synergy H1 microplate reader (Beckman Coulter, CA, USA).
6
Cell Viability Assay Using CCK8 Reagent
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Cells were seeded in to a 96-well plate at a density of 1500 cell/well and cultured in a humidified cell culture incubator for 0, 24, 48, 72 and 96 hours, respectively. Subsequently, 10 μL CCK8 reaction solution (Solarbio, CA1210, Beijing, China) was added to the cell culture at indicated time point and incubated for 1 hour in a humidified cell culture incubator. The light absorption value (OD value) in each condition was captured at 450 nm wavelength on a Synergy H1 microplate reader (Winooski, Vermont, USA).
7
Cell Proliferation Assay for NSCLC
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NSCLC cells infected with lentivirus carrying shRNA targeting FNDC1 or control shRNA were seeded in to a 96 -well plate at a density of 1500 cell/well and cultured in a humidified cell culture incubator for 0, 24, 48, and 72 h, respectively. Subsequently, 10 μL CCK8 reaction solution (Solarbio Science & Technology Co., Ltd., Beijing, China) was added to the cell culture at indicated time point and the cells were further incubated for 3 h in a humidified cell culture incubator. The medium was then removed, and samples in each well were dissolved in 150 μL DMSO for 15 min at 37°C. The optical value of each sample at 450 nm was recorded with a multifunctional microplate reader (BioTek Instruments, Winooski, VT, USA).
8
Cell Proliferation Assay with CCK-8
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Y79 and WERI-Rb1 cells with indicated treatments were seeded into a 96-well plate at a density of 2000 cells/well and cultured in a cell incubator for 0 h, 24 h, 48 h, 72 h. 10 μL CCK8 reaction solution (Solarbio, Beijing, China) was added to each well at indicated time point. The cell culture was incubated in cell incubator for 4 h, and the absorbance at 450 nm was measured by a microplate reader (Bio-Tek Instrument, Winooski) [23 (link)].
9
Cell Viability Assay Protocol
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After transfection, cells were seeded in to a 96-well plate at a density of 1500 cell/well and cultured in a humidified cell culture incubator for 0, 24, 48, and 72 h, respectively. 10 μL CCK8 reaction solution (Solarbio, CA1210, Beijing, China) was added to the cell culture at indicated time point and incubated for 2 h in a humidified cell culture incubator. The light absorption value (OD value) in each condition was captured at 450 nm wavelength on a Synergy H1 microplate reader (Winooski, Vermont, USA).
10
Cell Proliferation Assay Utilizing CCK-8
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48 hours after transfection, cells were seeded in to a 96 -well plate at a density of 1000 cell/well and cultured in a humidified cell culture incubator for 0, 24, 48, 72 and 96 hours, respectively. Subsequently, 10 μL CCK8 reaction solution (Solarbio, Japan) was added to the cell culture at indicated time point and incubated for 1 hour in a humidified cell culture incubator. The light absorption value (OD value) in each condition was captured at 450 nm wavelength.
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