Mouse anti spike s2 1a9

Manufactured by GeneTex

The Mouse anti-spike S2 (1A9) is a monoclonal antibody that targets the S2 subunit of the SARS-CoV-2 spike protein. The S2 subunit is responsible for the fusion of the viral membrane with the host cell membrane, which is a crucial step in the viral entry process. This antibody can be used for the detection and analysis of the SARS-CoV-2 spike protein in various applications, such as ELISA, Western blotting, and immunohistochemistry.

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Lab products found in correlation

Ab49900, by Abcam (2 mentions) Epr3861, by Abcam (2 mentions) Af933, by R&D Systems (2 mentions) Epr4435, by Abcam (2 mentions)

2 protocols using mouse anti spike s2 1a9

Antibodies Used in SARS-CoV-2 Research

Cited 1 time

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The following antibodies were used in this study: mouse anti‐spike S2 (1A9, Gene Tex), mouse anti‐p55/24 (ARP366, Centre for AIDS Reagents), goat anti‐ACE2 (AF933, R&D Systems), rabbit anti‐ACE2 (EPR4435, Abcam), rabbit anti‐TMPRSS2 (EPR3861, Abcam), mouse anti‐β‐actin (ab49900, Abcam), and mouse anti‐CD81 (2.131, a kind gift from Prof. Jane McKeating, University of Oxford). Anti‐spike mAbs have been previously described (Graham et al, 2021 (link)). Early pandemic convalescent serum was sourced from the Scottish National Blood Transfusion Service (SNBTS). Scottish blood donors provide informed consent for microbiological testing at their donations, made under SNBTS Blood Establishment Authorisation. Use of samples for this study was approved by the SNBTS Research and Sample Governance Committee. Ethical approval was obtained for the SNBTS anonymous archive – IRAS project number 18005.

Cantoni D., Murray M.J., Kalemera M.D., Dicken S.J., Stejskal L., Brown G., Lytras S., Coey J.D., McKenna J., Bridgett S., Simpson D., Fairley D., Thorne L.G., Reuschl A., Forrest C., Ganeshalingham M., Muir L., Palor M., Jarvis L., Willett B., Power U.F., McCoy L.E., Jolly C., Towers G.J., Doores K.J., Robertson D.L., Shepherd A.J., Reeves M.B., Bamford C.G, & Grove J. (2022). Evolutionary remodelling of N‐terminal domain loops fine‐tunes SARS‐CoV‐2 spike. EMBO Reports, 23(10), e54322.

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SARS-CoV-2 Antibody Characterization

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The following antibodies were used in this study: mouse anti-spike S2 (1A9, Gene Tex), mouse anti-p55/24 (ARP366, Centre for AIDS Reagents), goat anti-ACE2 (AF933, R& D Systems), rabbit anti-ACE2 (EPR4435, abcam), rabbit anti-TMPRSS2 (EPR3861, abcam), mouse anti--actin (ab49900, abcam), mouse anti-CD81 (2.131, a kind gift from Prof. Jane McKeating, University of Oxford), convalescent serum 20/130 (National Institute for Biological Standards and Control), mouse anti-RBD 12443 and 12444 (Native Antigen Company).

Dicken S.J., Murray M.J., Thorne L.G., Reuschl A.K., Forrest C., Ganeshalingham M., Muir L., Kalemera M.D., Palor M., McCoy L.E., Jolly C., Towers G.J., Reeves M.B, & Grove J. (2021). Characterisation of B.1.1.7 and Pangolin coronavirus spike provides insights on the evolutionary trajectory of SARS-CoV-2.. bioRxiv.

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