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Anti rabbit igg apc secondary antibody

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The Anti-rabbit IgG-APC secondary antibody is a laboratory reagent used to detect the presence of rabbit immunoglobulin G (IgG) in biological samples. It is conjugated with the fluorescent dye allophycocyanin (APC), which allows for the visualization and quantification of rabbit IgG-containing molecules in various experimental techniques, such as flow cytometry, immunohistochemistry, and Western blotting.

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Lab products found in correlation

Accuri c6, by BD (2 mentions) Cytofix cytoperm, by BD (2 mentions) Cd11c pe cy7, by BD (2 mentions) Cd8 pe cy7, by BD (2 mentions) Cd20 pe cy7, by BD (2 mentions) Cd4 percp cy5, by BD (2 mentions) Cd123 percp cy5, by BD (2 mentions)

Spelling variants of this product

Anti-IgG-APC (7 citations) Rabbit anti-IgG (3 citations)

2 protocols using anti rabbit igg apc secondary antibody

1

SARS-CoV-2 Detection in Tonsil TMNCs

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Frozen purified TMNCs were analyzed by flow cytometry after a 30-min staining at 4°C using antibodies for CD4 (PerCP-Cy5.5), CD8 (PE-Cy7), CD11c (PE-Cy7), CD14 (PerCP), CD20 (PE-Cy7), and CD123 (PerCP-Cy5.5) (BD Pharmingen). Cells were then washed, permeabilized, and fixed with BD Cytofix/Cytoperm. Intracellular SARS-CoV-2 was stained with rabbit anti-SARS-CoV-2 NP antibody, followed by anti-rabbit IgG-APC secondary antibody (BD Pharmingen). TMNCs from tonsils RT-qPCR negative for SARS-CoV-2 were used as negative controls. Cell preparations stained only with the IgG-APC secondary antibody were used for calibration of PE acquisition. Acquisitions were performed in fixed cells in a flow cytometer (BD Accuri C6; BD Biosciences) and then analyzed using FlowJo software (Tree Star).
de Lima T.M., Martins R.B., Miura C.S., Souza M.V., Cassiano M.H., Rodrigues T.S., Veras F.P., Sousa J.D., Gomes R., de Almeida G.M., Melo S.R., da Silva G.C., Dias M., Capato C.F., Silva M.L., Luiz V.E., Carenzi L.R., Zamboni D.S., Jorge D.M., Cunha F.D., Tamashiro E., Anselmo-Lima W.T., Valera F.C, & Arruda E. (2023). Tonsils are major sites of persistence of SARS-CoV-2 in children. Microbiology Spectrum, 11(5), e01347-23.
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2

SARS-CoV-2 Detection in Tonsil TMNCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen purified TMNCs were analyzed by flow cytometry after a 30-min staining at 4°C using antibodies for CD4 (PerCP-Cy5.5), CD8 (PE-Cy7), CD11c (PE-Cy7), CD14 (PerCP), CD20 (PE-Cy7), and CD123 (PerCP-Cy5.5) (BD Pharmingen). Cells were then washed, permeabilized, and fixed with BD Cytofix/Cytoperm. Intracellular SARS-CoV-2 was stained with rabbit anti-SARS-CoV-2 NP antibody, followed by anti-rabbit IgG-APC secondary antibody (BD Pharmingen). TMNCs from tonsils RT-qPCR negative for SARS-CoV-2 were used as negative controls. Cell preparations stained only with the IgG-APC secondary antibody were used for calibration of PE acquisition. Acquisitions were performed in fixed cells in a flow cytometer (BD Accuri C6; BD Biosciences) and then analyzed using FlowJo software (Tree Star).
de Lima T.M., Martins R.B., Miura C.S., Souza M.V., Cassiano M.H., Rodrigues T.S., Veras F.P., Sousa J.D., Gomes R., de Almeida G.M., Melo S.R., da Silva G.C., Dias M., Capato C.F., Silva M.L., Luiz V.E., Carenzi L.R., Zamboni D.S., Jorge D.M., Cunha F.D., Tamashiro E., Anselmo-Lima W.T., Valera F.C, & Arruda E. (2023). Tonsils are major sites of persistence of SARS-CoV-2 in children. Microbiology Spectrum, 11(5), e01347-23.
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