All OC cell lines were maintained in Dulbecco’s modified eagle medium (DMEM) medium (Corning) with 10% fetal bovine serum (FBS), 100 units/mL penicillin, and 100 μg/mL of streptomycin in humidified air with 5% CO2 at 37 °C. The NOF151-hTERT (NOF) cell is an immortalized normal fibroblast cell line, kindly provided by Professor Jinsong Liu (MD Anderson, TX, USA). NOF cells were maintained in MCDB 105 and 199 media (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 15% FBS (ThermoFisher, Waltham, MA, USA) and antibiotics [53 (link)]. LY was kindly provided by Eli Lilly (Indianapolis, IN, USA). For in vitro study, LY was dissolved in DMSO at 10 mM and kept at −20 °C. For in vivo experiment, LY was freshly formulated on the night before the treatment in 1% carboxymethylcellulose/0.5% sodium lauryl sulfate/0.085% povidone.
Dulbecco s modified eagle medium dmem medium
Manufactured by Corning
Sourced in United States
Dulbecco's Modified Eagle Medium (DMEM) is a cell culture medium formulated to support the growth and maintenance of various cell types. It is a widely used basal medium that provides essential nutrients, vitamins, and amino acids required for cell proliferation. DMEM is designed to maintain the optimal pH and osmotic balance necessary for cell viability and function.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mhcc 97h, by National Collection of Authenticated Cell Cultures (1 mentions)
Hcclm3, by National Collection of Authenticated Cell Cultures (1 mentions)
Bel 7404, by National Collection of Authenticated Cell Cultures (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
2 protocols using dulbecco s modified eagle medium dmem medium
1
Cultivation of OC and NOF cell lines
2
Human HCC Tissue and Cell Line Acquisition
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Matched pairs of cancerous and normal tissue from 50 patients with HCC were obtained from the Second Affiliated Hospital of Nantong University. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). This study was approved by Ethics Committee of the Second Affiliated Hospital of Nantong University (No. 2020KT014). The study mainly uses biological specimens obtained in previous clinical diagnosis and treatment, and is a secondary use of biological specimens, which is approved by the ethics committee without informed consent. The clinical characteristics of the HCC patients are summarized in Table 1 .
The human HCC cell lines (Bel-7404, HCCLM3, MHCC-97H, Hep G2, and Huh-7) and normal human hepatocytes LO2 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM) medium (product code: 11960044; Corning, Corning, NY, USA) supplemented with 10% fetal bovine serum (FBS) (product code: 10099141C; Corning, Corning, NY, USA) and 1% penicillin and streptomycin (product code: 15140122; Gibco, Thermo Fisher Scientific, Grand Island, NY, USA) in a humidified incubator at 37 ℃ with a 5% CO2 atmosphere.
The human HCC cell lines (Bel-7404, HCCLM3, MHCC-97H, Hep G2, and Huh-7) and normal human hepatocytes LO2 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM) medium (product code: 11960044; Corning, Corning, NY, USA) supplemented with 10% fetal bovine serum (FBS) (product code: 10099141C; Corning, Corning, NY, USA) and 1% penicillin and streptomycin (product code: 15140122; Gibco, Thermo Fisher Scientific, Grand Island, NY, USA) in a humidified incubator at 37 ℃ with a 5% CO2 atmosphere.
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