Nci h460 h460

Non-small cell lung cancer cells used in the experiments including NCI-H460 [H460] (ATCC^® HTB-177™, RRID: CVCL_0459), NCI-H292 [H292] (ATCC^® CRL-1848™, RRID: CVCL_0455), A549 (ATCC^® CCL-185™, RRID: CVCL_0023), and BEAS-2B (ATCC^® CRL-9609™, a normal human bronchial epithelium) and HCT116 (ATCC^® CCL-247™, a human colorectal carcinoma cell line) were obtained from the American Type Culture Collection (Manassas, VA, USA). Primary dermal papilla (DP) was purchased from Celprogen (Benelux, The Netherlands). H460, H292, and HCT116 were cultured in RPMI (Roswell Park Memorial Institute) 1640, whereas BEAS-2B, A549, and DP were cultured in DMEM (Dulbecco’s Modified Eagle Medium) supplemented with 10% fetal bovine serum (FBS), 100 units/mL penicillin/streptomycin and 2 mM L-glutamine. The cells were incubated in a 5% CO₂ environment at 37 °C. The cells that reached 70–80% confluence were used for the next experiments.

Non-small cell lung cancer cells used in the experiments including NCI-H460 [H460] (ATCC^® HTB-177™, RRID: CVCL_0459), NCI-H292 [H292] (ATCC^® CRL-1848™, RRID: CVCL_0455), NCI-H23 [H23] (ATCC^® CRL-5800™, RRID: CVCL_1547), and A549 (ATCC^® CCL-185™, RRID: CVCL_0023) were obtained from the American Type Culture Collection (Manassas, VA, USA). H460, H292, and H23 cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium, and A549 cells was grown in Dulbecco’s Modified Eagle Medium (DMEM). All cell culture mediums were supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 100 units/mL of each of penicillin and streptomycin. Cells were placed in a humidified atmosphere of 5% carbon dioxide (CO₂) at 37 °C.

TAM-inducible Gpx4-knockout mouse immortalized fibroblasts (referred to as Pfa1 cells) were reported previously^{18 (link)}. Genomic Gpx4 deletion can be achieved by TAM-inducible activation of Cre recombinase using the CreER^T2/loxP system. HT-1080, 786-O, A375, NCI-H460 (H460), MDA-MB-436, HT-29, B16F10, and 4T1 cells were purchased from ATCC. THP-1 cells were obtained from DSMZ). Human PBMC cells were purchased from Tebu-bio (cat. no. 088SER-PBMC-F). SUDHL5, SUDHL6, DOHH2 and OCI-Ly19 cells were a gift from S. Hailfinger. Rat1 cells were a gift from Medizinische Hochschule Hannover. Pfa1, HT-1080, 786-O, A375, HT-29, Rat1 and B16F10 cells were cultured in high-glucose DMEM (4.5 g l^–1 glucose) with 10% FBS, 2 mM l-glutamine and 1% penicillin-streptomycin. H460, MDA-MB-436, THP-1, PBMC, SUDHL5, SUDHL6, DOHH2 and 4T1 cells were cultured in RPMI GlutaMax with 10% FBS and 1% penicillin-streptomycin. OCI-Ly19 cells were cultured in IMDM with 10% FBS and 1% penicillin-streptomycin. To generate cell lines with stable overexpression, appropriate antibiotics (1 µg ml^–1 puromycin, 10 µg ml^–1 blasticidin and 0.5–1.0 mg ml^–1 G418) were used. GPX4-knockout human A375 and H460 cells were cultured in the presence of 1 µM Lip-1 for maintenance. All cells were cultured at 37 °C with 5% CO₂ and verified to be negative for mycoplasma.