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Lsm 780 nlo with examiner

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The LSM 780 NLO with Examiner is a multiphoton laser scanning microscope system designed for advanced live-cell and tissue imaging. It features a highly sensitive detector for detecting low-level fluorescence signals, enabling high-resolution imaging of thick samples. The system is equipped with a tunable femtosecond laser to provide excitation wavelengths suitable for a wide range of fluorophores.

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LSM 780 (3264 citations) LSM 780 NLO (183 citations)

3 protocols using lsm 780 nlo with examiner

1

Drosophila Brain Immunofluorescence Imaging

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For tau, piwi, and GFP immunofluorescence, Drosophila brains were dissected in PBS and fixed in methanol for 20 min. After blocking with 2% milk in 0.3% triton in PBS for 30 min, brains were incubated with primary antibody diluted in blocking solution overnight at 4°C. After washing with 0.3% triton in PBS, brains were incubated with Alexa488- or Alexa555-conjugated secondary antibodies for 2 h at room temperature. Slides were washed again and incubated with DAPI for 2 min to stain nuclei. Brains were visualized by confocal microscopy (Zeiss LSM 780 NLO with Examiner), and ImageJ was used for analysis. All images shown are a single slice. TUNEL staining was performed in 4 µm sections from formalin-fixed, paraffin embedded Drosophila heads. Secondary detection was performed with DAB. TUNEL-positive neurons were counted throughout the entire brain by bright field microscopy. Antibody concentrations and sources are listed in Supplementary Table 8.
Sun W., Samimi H., Gamez M., Zare H, & Frost B. (2018). Pathogenic tau-induced piRNA depletion promotes neuronal death through transposable element dysregulation in neurodegenerative tauopathies. Nature neuroscience, 21(8), 1038-1048.
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2

Drosophila Brain Immunofluorescence Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
For tau, piwi, and GFP immunofluorescence, Drosophila brains were dissected in PBS and fixed in methanol for 20 min. After blocking with 2% milk in 0.3% triton in PBS for 30 min, brains were incubated with primary antibody diluted in blocking solution overnight at 4°C. After washing with 0.3% triton in PBS, brains were incubated with Alexa488- or Alexa555-conjugated secondary antibodies for 2 h at room temperature. Slides were washed again and incubated with DAPI for 2 min to stain nuclei. Brains were visualized by confocal microscopy (Zeiss LSM 780 NLO with Examiner), and ImageJ was used for analysis. All images shown are a single slice. TUNEL staining was performed in 4 µm sections from formalin-fixed, paraffin embedded Drosophila heads. Secondary detection was performed with DAB. TUNEL-positive neurons were counted throughout the entire brain by bright field microscopy. Antibody concentrations and sources are listed in Supplementary Table 8.
Sun W., Samimi H., Gamez M., Zare H, & Frost B. (2018). Pathogenic tau-induced piRNA depletion promotes neuronal death through transposable element dysregulation in neurodegenerative tauopathies. Nature neuroscience, 21(8), 1038-1048.
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3

Drosophila Brain Immunohistochemistry

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Drosophila brains were dissected in PBS and fixed in 4% paraformaldehyde for 15 minutes. After blocking for 30 minutes with 2% milk in 0.03% TritonX in PBS, brains were incubated in primary antibody in 2% milk overnight at 4 C. After washing with 0.3% Triton in PBS, brains were incubated with Alexa488- or Alexa555-conjugated secondary antibodies for 2 h at room temperature. Slides were washed again and incubated with DAPI for 2 min to stain nuclei. Brains were visualized by confocal microscopy (Zeiss LSM 780 NLO with Examiner), and ImageJ was used for analysis. All images shown are a single slice.
Schulz L., Ramirez P., Lemieux A., Gonzalez E., Thomson T, & Frost B. (2022). Tau-induced elevation of the activity-regulated cytoskeleton associated protein Arc1 causally mediates neurodegeneration in the adult Drosophila brain. Neuroscience, 518, 101-111.
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