Anti slc1a5

Manufactured by Merck Group

Sourced in United States

Anti-SLC1A5 is a lab equipment product that targets the SLC1A5 protein, which is involved in the transport of amino acids. This product can be used for research purposes to study the function and regulation of the SLC1A5 protein.

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Lab products found in correlation

Pierce classic magnetic ip co ip kit, by Thermo Fisher Scientific (1 mentions) Anti cleaved caspase 6, by Cell Signaling Technology (1 mentions) Anti cleaved caspase 7, by Cell Signaling Technology (1 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Igg isotype control, by Cell Signaling Technology (1 mentions) Anti caspase 6, by Cell Signaling Technology (1 mentions) Anti caspase 7, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Anti ki67 sp6, by Thermo Fisher Scientific (1 mentions) Phospho gsk3α β ser21 9, by Cell Signaling Technology (1 mentions) Anti ttf1 8g7g3 1, by Agilent Technologies (1 mentions) Anti myc y69, by Abcam (1 mentions) Phospho s6, by Cell Signaling Technology (1 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) C jun, by Cell Signaling Technology (1 mentions) Scanscope at, by Leica (1 mentions)

2 protocols using anti slc1a5

Investigating Amino Acid Metabolism Regulation

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Cells were collected and lysed with lysis buffer supplemented with a protease and phosphatase inhibitor cocktail (Thermo Fisher, MA, USA). Then, the proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted. The antibodies used are as follows: anti-SLC1A5 (SAB2108565, Sigma, MO, USA), anti-SLC7A5 (PA5-50485, Thermo Fisher, MA, USA), anti-glutamine (ab93434, Abcam, UK), anti-glutamate dehydrogenase (PA5-28301, Thermo Fisher, MA, USA), anti-caspase 3, anti-cleaved caspase 3, anti-caspase 6, anti-cleaved caspase 6, anti-caspase 7, anti-cleaved caspase 7, and anti-β-actin (Cell Signaling Technology, MA, USA). Secondary antibodies conjugated to horseradish peroxidase were subsequently incubated with the membranes, followed by enhanced chemiluminescence development (Thermo Fisher, MA, USA). Three independent experiments were performed for each analysis. Co-immunoprecipitation was performed using the Pierce Classic Magnetic IP/Co-IP Kit (Thermo Fisher, MA, USA). Anti-AhR (Abcam, Ab2769) and IgG isotype control (Cell Signaling Technology, 3900) antibodies were used.

Shi J., Ju R., Gao H., Huang Y., Guo L, & Zhang D. (2021). Targeting glutamine utilization to block metabolic adaptation of tumor cells under the stress of carboxyamidotriazole-induced nutrients unavailability. Acta Pharmaceutica Sinica. B, 12(2), 759-773.

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Tissue Analysis of Lung Tumors

Cited 3 times

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Tissue isolation, fixation and staining procedures were performed as previously described (Momcilovic et al., 2017 (link); Momcilovic et al., 2015 (link); Shackelford et al., 2013 (link)). Briefly, lungs or tumors were fixed overnight in 10% buffered formalin, and then transferred to 70% ethanol. Tissues were processed and embedded by TPCL at UCLA. The following antibodies were used: phospho-4EBP1 (Thr37/46) (Cell Signaling Technology, #2855 1:800), anti-CK5 (EP1601Y) (abcam, ab52635 1:100), anti-TTF1 (8G7G3/1) (Dako, 1:1000), anti-Ki67 (SP6) (Thermo Scientific, RM-9106-S0 1:200), phospho-S6 (Cell Signaling Technology, #4585 1:400), phospho-GSK3α/β (Ser21/9) (Cell Signaling Technology, #9331 1:50), c-Jun (Cell Signaling Technology, #9165 1:800), phospho-c-Jun (S73) (D47G9) (Cell Signaling Technology, #3270 1:200), anti-SLC1A5 (Sigma, HPA035240, 1:400), anti-GLUT1 (Alpha Diagnostic, GT11A, 1:400), anti-Myc (Y69) (abcam, ab32072, 1:100). Slides were scanned onto a ScanScope AT (Aperio Technologies, Inc., Vista, CA). Digital slides were analyzed with QuPath software in order to determine percent positive cells for Ki67, GLUT1 and p4EBP1 stains.

Momcilovic M., Bailey S.T., Lee J.T., Fishbein M.C., Braas D., Go J., Graeber T.G., Parlati F., Demo S., Li R., Walser T.C., Gricowski M., Shuman R., Ibarra J., Fridman D., Phelps M.E., Badran K., John M.S., Bernthal N.M., Federman N., Yanagawa J., Dubinett S.M., Sadeghi S., Christofk H.R, & Shackelford D.B. (2018). The GSK3 signaling axis regulates adaptive glutamine metabolism in lung squamous cell carcinoma. Cancer cell, 33(5), 905-921.e5.

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