Leaf samples were homogenised in 250 mM Tris HCl (pH 7.8), mixed with equal volume of Laemmli buffer (2x), boiled for 5 min, clarified by centrifugation and separated on a 12% SDS polyacrylamide gel. Each sample was comprised of three discs obtained from the different agroinfiltrated leaves of one plant. Coomassie staining was performed by Coomassie blue. The scFv-TM43-E10 blots were probed with an anti-c-Myc mouse antibody (Sigma-Aldrich). The scFv-Fc-TM43-E10 membranes were developed using an anti-human lgG (Fc) mouse antibody (Sigma-Aldrich). Cross-reacting protein bands with secondary anti-mouse goat antibody (Sigma-Aldrich) were visualized with BCIP/NBT color developing reagent.
Anti mouse goat antibody
Manufactured by Merck Group
The anti-mouse goat antibody is a laboratory reagent used in various immunological techniques. It is a polyclonal antibody produced in goats that specifically binds to mouse antibodies or mouse-derived proteins. This antibody can be used to detect, quantify, or purify mouse-specific targets in samples.
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Lab products found in correlation
2 protocols using anti mouse goat antibody
1
Protein Expression Analysis Protocol
2
Rabies Virus Infection in BHK-21 Cells
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BSR cells (clone of BHK-21) were maintained in Dulbecco's Modified Eagle Medium (DMEM) with 8% Fetal Bovine Serum (FBS), gentamycin 40 µg/ml, 5% CO2 in 96 well plates. BHK-T7 were maintained in MEM-Glasgow with 10% FBS supplemented with 4%
Tryptose Phosphate Broth (TPB) and Pennicillin (100U/ml) / Streptomycin (100µg/ml). The Pasteur Virus (PV) strain of RABV was used for infection experiments.
The following anti-RABV antibodies have been used: a mouse monoclonal antibody (MAb) anti-N conjugated with FITC (Fudjerebio); a rabbit polyclonal antibody (PolAb) anti-RNP and a mouse MAb anti-G protein (D1), both produced in the laboratory (Tuffereau et al., 2001; Fournier et al., 2003) ; a mouse MAb anti-M protein kindly provided by S. Finke (Friedrich Loeffler Institute, Griefswald, Germany). A mouse MAb anti-calnexin, a mouse MAb anti-tubulin, a rabbit PolAb anti-Rab7a, an anti-rabbit goat antibody and an anti-mouse goat antibody were purchased (Sigma).
Tryptose Phosphate Broth (TPB) and Pennicillin (100U/ml) / Streptomycin (100µg/ml). The Pasteur Virus (PV) strain of RABV was used for infection experiments.
The following anti-RABV antibodies have been used: a mouse monoclonal antibody (MAb) anti-N conjugated with FITC (Fudjerebio); a rabbit polyclonal antibody (PolAb) anti-RNP and a mouse MAb anti-G protein (D1), both produced in the laboratory (Tuffereau et al., 2001; Fournier et al., 2003) ; a mouse MAb anti-M protein kindly provided by S. Finke (Friedrich Loeffler Institute, Griefswald, Germany). A mouse MAb anti-calnexin, a mouse MAb anti-tubulin, a rabbit PolAb anti-Rab7a, an anti-rabbit goat antibody and an anti-mouse goat antibody were purchased (Sigma).
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