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Dip 360 polarimeter

Manufactured by Jasco
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Sourced in Japan

The DIP-360 polarimeter is a laboratory instrument used to measure the optical rotation of a substance. It determines the degree of rotation of polarized light as it passes through a sample. The DIP-360 provides accurate and reliable measurements of the optical activity of various materials, which is a fundamental property in many scientific and industrial applications.

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Lab products found in correlation

Jms hx110 mass spectrometer, by JEOL (1 mentions) 60f254 silica gel, by Merck Group (1 mentions) Eca 600 spectrometer, by JEOL (1 mentions) 230 400 mesh silica gel, by Merck Group (1 mentions) Av 400 spectrometer, by Bruker (1 mentions) Ultraflextreme maldi tof tof mass spectrometer, by Bruker (1 mentions) Jms hx 110, by JEOL (1 mentions) 400 mhz spectrometer, by Agilent Technologies (1 mentions) Mercury 400 mhz spectrometer, by Agilent Technologies (1 mentions) Onenmr probe, by Agilent Technologies (1 mentions) Jms 700 mstation, by JEOL (1 mentions) Hx 110 mass spectrometer, by JEOL (1 mentions) Silica gel 60, by Merck Group (1 mentions) Melting point apparatus, by Gallenkamp (1 mentions) Melting point m 560 apparatus, by Büchi (1 mentions) 320 a spectrophotometer, by Jasco (1 mentions) Uv 3200 spectrophotometer, by Hitachi (1 mentions)

Close spelling variants of this product

DIP-360 digital polarimeter DIP-360

7 protocols using dip 360 polarimeter

1

Analytical Characterization of Compound 1

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Optical rotation for compound 1 was measured on a DIP 360 polarimeter (JASCO, Tokyo, Japan). IR spectrum was recorded on a spectrophotometer with ATR-Tensor 37 by Bruker. JMS HX 110 Mass spectrometer (JEOL, Freising, Germany) was used to acquire the mass spectra. 1H and 13C NMR spectra were recorded on AM 600 NMR spectrometer (Bruker, Fallanden, Switzerland), and the chemical shift values were recorded in ppm (δ) units, whereas the coupling constants (J) were reported in Hz. The Final purification of compound 1 was carried out by using recycling HPLC (JAI), and chloroform was used as the mobile phase in a series of two silica columns; JAIGEL-1H and JAIGEL-2H (Serial No. J60-3E13, JAI, Yokohama, Japan). The TLC experiments were carried out on aluminum sheets pre-coated with 60F-254 silica gel (E. Merck, Darmstadt, Germany), which were developed in a mobile phase of 1% MeOH/dichloromethane to 5% MeOH/dichloromethane. The developed TLC plates were then sprayed in ceric sulphate and heated to observe the UV-inactive compounds.
Ali L., Khan A.L., Al-Broumi M., Al-Harrasi R., Al-Kharusi L., Hussain J, & Al-Harrasi A. (2017). New Enzyme-Inhibitory Triterpenoid from Marine Macro Brown Alga Padina boergesenii Allender & Kraft. Marine Drugs, 15(1), 19.
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2

Characterization of Natural Products

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Optical rotations were measured on a Jasco DIP-360 polarimeter. UV spectrum was obtained on a Jasco V-550 spectraphotometer (Tokyo, Japan). FAB-MS and HR-FAB-MS were obtained using a JEOLJMS-GC mate spectrometer (JEOL, Tokyo, Japan). NMR spectra were recorded using a JEOL ECA 600 spectrometer (JEOL, Tokyo, Japan), with TMS as an internal standard. Silica gel (230–400 mesh, Merck, Darmstadt, Germany) and Silica gel plates (GF254, Merck, Darmstadt, Germany) were used for CC and TLC, respectively. Sephadex LH-20 was purchased from Pharmacia (Uppsala, Sweden). ODS-EP column (250 × 10 mm, Inertsil; GL sciences Inc., Tokyo, Japan) was used for preparative HPLC.
Sun Y., Gao L.L., Tang M.Y., Feng B.M., Pei Y.H, & Yasukawa K. (2018). Triterpenoids from Euphorbia maculata and Their Anti-Inflammatory Effects. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(9), 2112.
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3

Synthetic Procedures and Characterization of Organic Compounds

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All reagents were purchased from commercially sources and were used without further purification. All solvents were available with commercially dried or freshly dried and distilled prior to use. Reactions were monitored by thin layer chromatography (TLC) using silica gel GF254 plates with detection by short wave UV light (λ = 254 nm) and staining with 10% phosphomolybdic acid in EtOH or p–anisaldehyde solution (ethanol/p–anisaldehyde/acetic acid/sulfuric acid 135:5:4:1.5), followed by heating on a hot plate. Column chromatography was conducted by silica gel (200–300 mesh) with ethyl acetate and hexane as eluent. Optical rotation values were measured using a JASCO DIP-360 polarimeter at the ambient temperature in specified solvents. 1H NMR and 13C NMR were recorded with Bruker AV 400 spectrometer at 400 MHz (1H NMR), 100 MHz (13C NMR) using CDCl3 and CD3OD as solvents. Chemical shifts were reported in δ (ppm) from CDCl3 (7.26 ppm for 1H NMR, 77.00 ppm for 13C NMR), CD3OD (3.31 ppm for 1H NMR, 49.00 ppm for 13C NMR). Coupling constants were reported in hertz. High–resolution mass spectra (HRMS) were obtained on a Varian QFT–ESI mass spectrometer, and glycoproteins were analyzed by Bruker ultrafleXtreme MALDI TOF/TOF mass spectrometer.
Shang W., Xiao Z., Yu Z., Wei N., Zhao G., Zhang Q., Wei M., Wang X., Wang P.G, & Li T. (2015). Chemical Synthesis of Outer Core Oligosaccharide of Escherichia coli R3 and Immunological Evaluation. Organic & biomolecular chemistry, 13(14), 4321-4330.
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4

Spectroscopic Analysis of Isolated Compounds

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The optical rotations ([α]25D) were obtained through a “JASCO DIP 360 polarimeter (Tokyo, Japan)” while the melting points (mps) were measured using “BioCote Stauart SMP10 (Tokyo, Japan)” melting point instrument. The mass spectral assignments were made from EI-MS/HR-EIMS spectra obtained through “JEOL JMS HX 110 (Tokyo, Japan)” while 1H-NMR/13C-NMR spectral measurements were carried out by using “Bruker NMR, Germany (500, 600 MHz for 1H-NMR; 125, 150 MHz for 13C-NMR (δ, ppm)), respectively. FT-IR analyses were determined on “JASCO-320-A spectrophotometer in KBr” as well as “Perkin–Elmer spectrophotometer”. All the solvents used in extraction and isolation of compounds were distilled before use, while the deuterated solvents were used for NMR analysis. Thin layer chromatography (TLC) was carried out using “silica gel F254 pre-coated aluminum sheets”. Visualization of TLC was conducted through a UV lamp at both 254 & 366 nm (λmax) as well as “Dragendorff’s reagent”. The solvent system; 20% acetone-hexane: 10 drops of diethylamine was used as developing solvent for TLC.
Ahmad S., Ahmad M., Almehmadi M., Shah S.A., Khan F.A., Khan N.M., Khan A., Z.a.i.n.a.b., Halawi M, & Ahmad H. (2022). In Vitro and In Silico Investigation of Diterpenoid Alkaloids Isolated from Delphinium chitralense. Molecules, 27(14), 4348.
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5

Spectroscopic Characterization of Organic Compounds

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Infrared absorbance spectra were collected with a Fourier transform infrared (FTIR) spectrometer (NICOLET 6700). Positive FAB-MS spectra were recorded on a JEOL MStation JMS700 mass spectrometer using m-nitrobenzyl alcohol as the matrix. 1H, gCOSY, and HSQC NMR experiments were performed with a Varian Mercury 400 MHz spectrometer. 13C experiments were performed with a Varian 400 MHz spectrometer equipped with a Varian OneNMR probe. Chemical shifts were referenced to the residual solvent peaks in CD3OD. Optical rotation was measured with a Jasco DIP 360 polarimeter fitted with a microcell (10 mm path length).
Andrade-Domínguez A., Trejo-Hernández A., Vargas-Lagunas C, & Encarnación-Guevara S. (2021). Phenotypic plasticity and a new small molecule are involved in a fungal-bacterial interaction. Scientific Reports, 11, 19219.
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6

Physicochemical Characterization of Compounds

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The melting points were measured on a Gallenkamp melting point apparatus. Optical rotations were recorded with a JASCO DIP-360 polarimeter. The IR spectrum was measured in MeOH on a JASCO A-302 spectrophotometer. The proton and carbon (1D and 2D)-NMR (600/500 MHz) spectra were measured on a Bruker AMX machine. The chemical shifts of proton and carbon were recorded based on the internal reference TMS (Tetramethylsilane) in δ (ppm). Moreover, coupling constants (J) were measured in Hz. The ESIMS was recorded on a double-focusing mass spectrometer (Varian MAT 311A), while HREIMS was recorded on a JEOL HX 110 mass spectrometer. Silica gel 60 (0.2–0.5 mm and 0.2–0.063 mm) (70–230 and 240–300 mesh sizes, E. Merck) was used as a stationary phase for CC (Column Chromatography). The purity of compounds and the monitoring of fractions were based on precoated silica gel TLC (Thin Layer Chromatography) plates supported on either plastic or aluminum sheets (E. Merck, F254). Spots were visualized on TLC with UV light (254 nm and 365 nm) on a CN-6 UV spectrometer (made in France) then sprayed with ceric sulphate and heated at about 90 °C.
Leutcha B.P., Dzoyem J.P., Jouda J.B., Sema D.K., Tsague Tankeu V.F., Bitchagno G.T., Tchegnitegni B.T., Essoung F.R., Ndjakou Lenta B., Fogue Kouam S., Delie F., Meli Lannang A, & Sewald N. (2022). Antimicrobial and Cytotoxic Activities of Constituents from the Fruit of Albizia lebbeck L. Benth (Fabaceae). Molecules, 27(15), 4823.
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7

Analytical Characterization of Compounds

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Melting points were measured on a Büchi M-560 melting point apparatus. Optical rotations were obtained with a JASCO DIP-360 polarimeter. UV spectra were recorded on a Hitachi UV 3200 spectrophotometer. A JASCO 320-A spectrophotometer was used for scanning IR spectroscopy using KBr pellets. 1D and 2D NMR spectra of compounds 1-4 recorded on a Bruker AM spectrometer operating at 150 and 600 MHz, respectively where chemical shifts (δ) were expressed in ppm with reference to the TMS. EI-MS spectra were obtained on Varian MAT 311A mass spectrometer operating at 300 °C. HPLC was carried out on recycling preparative High Performance Liquid Chromatogram of Japan Analytical Industry ltd. (Model LC-90 W). The column was of silica D-60 (internal diameter 20 mm, length 250 mm, particle size 40 µm. UV detector (Model 310) was used for detection.
Bankeu J.J., Sattar H., Fongang Y.S., Muhammadi S.W., Simoben C.V., Ntie-Kang F., Feuya G.R., Tchuenmogne M.A., Lateef M., Lenta B.N., Ali M.S, & Ngouela A.S. (2018). Synthesis, Urease Inhibition and Molecular Modelling Studies of Novel Derivatives of the Naturally Occurring β-Amyrenone. Natural Products and Bioprospecting, 9(1), 49-59.
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