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5-HMF is a chemical compound that serves as a precursor for the synthesis of various organic compounds. It is commonly used in research and analytical applications. The core function of 5-HMF is to provide a starting material for chemical reactions and analyses without making any claims about its intended use.

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2 protocols using 5 hmf

1

Simultaneous Quantification of Phytochemicals in Dengue Medicine

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The HPLC and LCMS grade solvents acetonitrile and methanol were obtained from J. T. Baker (USA) and Honeywell (Germany). Analytical grade solvents, glacial acetic acid, and formic acid, toluene and ethyl acetate were purchased from Rankem, India. Deionized water, purified by a Milli-Q system (Millipore, USA), was used throughout the study. Reference standards with defined purity in parenthesis, for gallic acid (97.3%), magnoflorine (99.0%), berberine (88.4%), ellagic acid (99.6%), rosmarinic acid (98.0%) were procured from Sigma Aldrich (USA). 5-HMF (97.3%), methyl gallate (99.5%) from Tokyo Chemical Industries (Japan) and protocatechuic acid (99.5%), rutin (98.7%) from Natural Remedies (India) and β-ecdysone (99.9%) were purchased from PHY-proof (Germany), respectively. Divya-Denguenil-Vati (DNV) batches were sourced from Divya Pharmacy, Haridwar (India), and were stored in airtight bottles. DNV (batch # 015) was used for UPLC/MS-QToF identification, as well as UHPLC and HPTLC validation studies. Twenty-six commercial batches of DNV, # 014, # 015, # 029 to # 034, and # 036 to # 053 were analyzed to evaluate quality consistency across different DNV batches.
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2

Cellulose Hydrolysis and Characterization

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5‐HMF (>95.0 %, GC) and dimethyl sulfone (DMSO2, >99.0 %, GC) were purchased from Tokyo Chemical Industry, Tokyo, Japan. Before experiments, water was removed from 5‐HMF by freeze‐drying. Whatman No. 42 filter paper was used as a cellulose sample, which was cut into small sheets (7×7 mm). Glycerol (>99.5 %, GC), Na2CO3 (>99.5 %, titration) and NaHCO3 (99.5 %, titration) were purchased from Nacalai Tesque, Inc., Kyoto, Japan, and used without purification. Aqueous NaOH solution (47–53 %, T) and aqueous HCl solution (35–37 %, T) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and used without purification.
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