Lna934v

PrP^C-specific antibodies 3F4 and 8H4 were obtained from Signet laboratories (Dedham, MA, USA). 3F4 binds to an epitope on amino acids 109–112 (Human PrP sequence), while 8H4 binds an epitope in the C-terminal amino acids 145–180 of human and mouse PrP^{62 (link)}. Other primary antibodies were obtained from the following sources: anti-ferritin (F5012, Sigma Aldrich, USA), anti-DMT-1 (ab55812, Abcam, USA), anti-TfR (ab84036, Abcam, USA), anti-Tf (GTX21223, GeneTex, USA), and anti-β-actin (MAB1501, Millipore, USA) Secondary antibodies conjugated with HRP were from GE Healthcare (anti-mouse, LNA931V, anti-rabbit, LNA934V). Alexa fluor-conjugated secondary antibodies were from molecular probes, ImmPACT DAB (SK4105) from Vector labs, USA, PNGase F (P0704S) from NEB, USA and Lipofectamine 3000 transfection reagent from Invitrogen, USA. Radiolabeled ⁵⁹FeCl₃ was obtained from Perkin-Elmer, USA. siRNA against PrP (sc36318) and scrambled siRNA (sc37007) were purchased from Santa Cruz Biotechnology Inc, USA. For immunohistochemistry of hamster retinal sections PrP-6C2 (CVI-WUR, Lelystad, Netherland), ferritin (Jackson ImmunoResearch, West Grove, PA, USA), and Iba1 (019–19741, Wako Chemicals, USA) antibodies were used. FAC (F5879) and all other chemicals were from Sigma Aldrich, USA.

The following antibodies were used: mAB anti‐phospho‐CDK substrate (pTPXK, rabbit, Cell Signalling, 14371), mAB anti‐phospho‐CK2 substrate MultiMab™ ((pS/pT)DXE, rabbit, Cell Signalling, 8738), mAB anti‐phospho‐ MAPK/CDK substrate (PXS*P or SPXR/K, rabbit, Cell Signalling, 2325), mAB anti‐phospho‐PKA substrate (RRXS*/T*, rabbit, Cell Signalling, 9624), pAB anti‐phospho‐(Ser)‐PKC substrate ((R/K)X(S*)(Hyd)(R/K), rabbit, Cell Signalling, 2261), pAB anti‐GST (goat, GEHealthcare, 27457701V), mAB anti‐HA (mouse, Hiss Diagnostics GmbH, AB‐10110), mAB anti‐phospho‐tyrosine clone 4G10 (mouse, Millipore, 05‐321), secondary mAB anti‐goat HRP (rabbit, Invitrogen, 611620), secondary mAB anti‐mouse (sheep, GE Healthcare, LNA931V), secondary mAB anti‐rabbit (donkey, GE Healthcare, LNA934V).