2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) was purchased from Sigma‐Aldrich, Germany. Folin–Ciocalteau reagent was purchased from Merck Specialities Private Limited, India. Gallic acid was purchased from LOBA Chemie, India. Methanol was purchased from Fisher scientific, India. PCA agar, PDA agar, MRS agar, and VRBA agar media were purchased from Hi‐Media Laboratories, India. The spectrophotometer used was of Model GENESYSTM 10S Vis spectrophotometer from Thermo Scientific TM, Germany.
Pca agar
Manufactured by HiMedia
Sourced in India
PCA agar is a general-purpose culture medium used for the growth and enumeration of a wide variety of microorganisms. It provides the necessary nutrients and growth factors for the proliferation of both aerobic and facultative anaerobic bacteria.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Folin ciocalteau reagent, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Gallic acid, by Loba Chemie (1 mentions)
Pda agar, by HiMedia (1 mentions)
Mrs agar, by HiMedia (1 mentions)
Folin ciocalteu s phenol reagent, by Merck Group (1 mentions)
Carrageenan, by Merck Group (1 mentions)
M ller hinton broth, by HiMedia (1 mentions)
Peptone, by HiMedia (1 mentions)
3 protocols using pca agar
1
DPPH Antioxidant Assay Protocol
2
Carrageenan-Based Antioxidant Film Development
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The carrageenan was purchased from Sigma-Aldrich (St. Louis, MO, USA), where the type was κ-carrageenan. The plasticizer used was glycerol, which was purchased from Mistura da Terra (Bagé, Brazil). The following reagents were bought from Sigma-Aldrich (St. Louis, MO, USA) and were of an analytical standard: Folin Ciocalteu’s phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), methanol, anhydrous sodium carbonate, gallic acid, and oleuropein. For microbiological analyses: nutrient broth, Müller–Hinton broth, PCA agar, and peptone (Himedia, Bengaluru, India), as well as sterilized distilled water, were used.
3
Bacterial Enumeration via Dilution Plating
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The sample was diluted as 10−1, 10−2, 10−3, 10−4, 10−5, and 10−6 and 0.1 ml of each dilution was spread on plate count agar (PCA) agar (HiMedia, India) for 42 h at 37°C ± 1°C. All the colonies grown on the plates were counted.
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