Ym 08

PGE₁ and the mouse IL-6 enzyme-linked immunosorbent assay (ELISA) kit were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). VER-155008 and YM-08 were obtained from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). PD98059, SB203580, and SP600125 were obtained from Calbiochem-Novabiochem Co. (La Jolla, CA, USA). Phospho-specific p38 MAPK antibodies, p38 MAPK antibodies, and actin were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The enhanced chemiluminescence (ECL) Western blotting detection system was purchased from GE Healthcare Life Sciences (Chalfont, UK). Acrylamide monomer, Tris (hydroxymethyl) aminomethane, sodium dodecyl sulfate (SDS), dithiothreitol, and glycerol were obtained from Nacalai Tesque, Inc. (Kyoto, Japan). Other materials and chemicals were obtained from commercial sources. VER-155008, YM-08, PD98059, SB203580, and SP600125 were dissolved in dimethyl sulfoxide. The maximum concentration of dimethyl sulfoxide was 0.1%, which did not affect the IL-6 assay or protein level detection via Western blotting [19 (link), 23 (link), 24 (link)].

IGF-I was obtained from R&D System, Inc. (Minneapolis, MN, USA). VER-155008 and YM-08 were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Phospho-specific p44/p42 MAP kinase, p44/p42 MAP kinase, phospho-specific Akt (Thr308), and Akt antibodies were used for the first antibodies (Cell Signaling, Beverly, MA, USA). An ECL Western blotting detection kit was used (GE Healthcare UK Ltd., Buckinghamshire, UK). Other materials were purchased from commercial sources. VER-155008 and YM-08 were dissolved in dimethyl sulfoxide (DMSO). The maximum concentration of DMSO was 0.1%, which did not affect the assay for cell migration and the detection of the protein level using Western blotting.