The 96 micropillar–well plates were coated with poly-l -lysine (100 μg ml−1) for 1 h, washed twice with water and air dried. In each well, 40,000 rat OPC cells were seeded and maintained in chemically defined medium composed of DMEM (Invitrogen) supplemented with B27 (Invitrogen), N2 (Invitrogen), penicillin-streptomycin (Invitrogen), N-acetylcysteine (Sigma-Aldrich), forskolin (Sigma-Aldrich) and 12.5 ng ml−1 PDGF-AA (Peprotech) for 2 d before screening. Compounds (10 mM in DMSO, Chemical library, Selleckchem) were diluted to a final concentration of 1 μM in the absence of PDGF-AA and added to wells for 3 d before immunostaining. Each compound was tested in triplicate.
Pdgf aa
Manufactured by Selleck Chemicals
Sourced in United States
PDGF-AA is a recombinant human protein that functions as a growth factor. It is a member of the platelet-derived growth factor family and plays a role in cellular processes such as cell growth, cell migration, and angiogenesis.
Automatically generated - may contain errors
Lab products found in correlation
Forskolin, by Merck Group (1 mentions)
Pdgf aa, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
Crenolanib, by Selleck Chemicals (1 mentions)
Igf 1, by Thermo Fisher Scientific (1 mentions)
Sdf 1α, by R&D Systems (1 mentions)
Pdgf dd, by R&D Systems (1 mentions)
Pdgf aa, by R&D Systems (1 mentions)
Bmp 2, by Medtronic (1 mentions)
Pdgf ab, by R&D Systems (1 mentions)
Pdgf cc, by R&D Systems (1 mentions)
Pdgf bb, by R&D Systems (1 mentions)
Hepatocyte growth factor (hgf), by Thermo Fisher Scientific (1 mentions)
Tgf β3, by Miltenyi Biotec (1 mentions)
2 protocols using pdgf aa
1
Screening OPC Cells for Compound Effects
2
Chemotactic migration of cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We used recombinant human bone morphogenetic protein-2 (BMP-2; Medtronic, Minneapolis, MN, USA), hepatocyte growth factor (HGF; Peprotech, Rocky Hill, NJ, USA), insulin-like growth factor-1 (IGF-1; Peprotech), platelet-derived growth factor-AA (PDGF-AA; R&D Systems), PDGF-AB (R&D Systems), PDGF-BB (R&D Systems), PDGF-CC (R&D Systems), PDGF-DD (R&D Systems), stromal cell-derived factor-1α (SDF-1α; R&D Systems), transforming growth factor-β3 (TGF-β3; Miltenyi Biotec, Bergisch Gladbach, Germany). The medium containing 0.5% FBS in the lower chamber was supplemented with 100 ng/mL BMP-2, 100 ng/mL HGF, 100 ng/mL IGF-1, 10 ng/mL PDGF-BB, 100 ng/mL SDF-1α, or 10 ng/mL TGF-β3. After 48 h incubation, migrated cells were counted. The PDGF isoforms were compared by adding PDGF-AA, AB, BB, CC, or DD to the lower chamber at 100 ng/mL. Crenolanib (Selleck Chemicals, Houston, TX, USA), an inhibitor of PDGF receptor (PDGFR) α and β, was added to both the upper and lower chambers during the migration assay.
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