Resuspended SK-BR-3 breast, T24 bladder, and SK-Mel-28 melanoma cancer cells were washed and resuspended in 1% BSA-PBS to achieve a cell concentration of 1 × 106 cells/ml. Cell mixtures were incubated with PE conjugated anti-EpCAM (BD Biosciences, San Jose, CA, USA) or IgG2b as a negative control (0.5 μg/test) at room temperature for 15 min. Cells were washed and resuspended in 1 ml of 1% BSA in PBS containing 7-Aminoactionomycin D (7-AAD) (Life Technologies, Carlsbad, CA, USA), followed by flow cytometric analysis on the gated live cells.
Pe conjugated anti epcam
Manufactured by BD
Sourced in United States
PE conjugated anti-EpCAM is a monoclonal antibody that binds to the Epithelial Cell Adhesion Molecule (EpCAM). EpCAM is a cell surface glycoprotein expressed on epithelial cells and various types of cancer cells. The antibody is conjugated with the fluorescent dye Phycoerythrin (PE), which allows for the detection and analysis of EpCAM-expressing cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
7 aminoactionomycin d 7 aad, by Thermo Fisher Scientific (1 mentions)
Apc conjugated anti cd56, by Miltenyi Biotec (1 mentions)
Fitc conjugated anti cd90, by BD (1 mentions)
Canto 2 instrument, by BD (1 mentions)
Foxp3 staining buffer kit, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti cd107a, by BD (1 mentions)
Fitc conjugated anti cd44, by BD (1 mentions)
Bv510 conjugated anti cd3, by BD (1 mentions)
Live dead red fluorescent reactive dye, by Thermo Fisher Scientific (1 mentions)
2 protocols using pe conjugated anti epcam
1
EpCAM Expression on Cancer Cells
2
Multi-Color Flow Cytometry for Cell Characterization
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The following commercially available antibodies were used for multi-color flow cytometry: BV510-conjugated anti-CD3, PE-conjugated anti-CD107a, APC-conjugated anti-ICAM1, PE-conjugated anti-EpCAM, PE-conjugated anti-CD13, FITC-conjugated anti-CD44, FITC-conjugated anti-CD90, PE-conjugated anti-CD133 (BD Biosciences, San Jose, CA, USA), APC-conjugated anti-CD56 (Miltenyi Biotec), APC-conjugated anti-MHC-1, APC-conjugated anti-ULBP-1, APC-conjugated anti-ULBP-2/5/6, APC-conjugated anti-ULBP-3, APC-conjugated anti-TRAIL-R1 (R&D Systems, Minneapolis, MN, USA), APC-conjugated anti-CEACAM1, APC-conjugated anti-HLA-G, and APC-conjugated anti-MICA/B (Biolegend, San Diego, CA, USA). Dead cells were excluded using the LIVE/DEAD red fluorescent reactive dye (Invitrogen). For some experiments, surface marker–stained cells were permeabilized using a Foxp3 Staining Buffer Kit (eBioscience) and further stained for PE-conjugated anti-aldehyde dehydrogenases (ALDH) (Sino Biological, PA, USA). Multi-color flow cytometry was performed using the Canto II instrument (BD Biosciences), and data were analyzed using the FlowJo software (TreeStar, Ashland, OR, USA).
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