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Tecnai 20 feg electron microscope

Manufactured by Thermo Fisher Scientific
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The Tecnai 20 FEG is a high-resolution electron microscope manufactured by Thermo Fisher Scientific. It is equipped with a field emission gun (FEG) that provides a high-brightness electron beam for advanced imaging and analytical capabilities. The Tecnai 20 FEG is designed for materials science, life science, and nanotechnology research applications.

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Lab products found in correlation

Carbon coated 400 mesh cu rh grid, by Ted Pella (2 mentions) Tecnai 12, by Thermo Fisher Scientific (2 mentions) Leica em gp, by Leica Microsystems (2 mentions)

2 protocols using tecnai 20 feg electron microscope

1

Dynamin Interactions with Liposomes Visualized by Electron Microscopy

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For negative stain EM, samples (1-3 μM dynamin and 200 μM DOPS liposomes incubated in the presence or absence of 1 mM GTP or GMPPCP for 30 min at room temperature) were absorbed onto carbon-coated 400 mesh Cu/Rh grids (Ted Pella, Inc.), stained with 2% Uranyl Acetate, and imaged in a Tecnai 12 (FEI) transmission electron microscope at 120kV using a 2 × 2 Gatan CCD camera. For cryo-EM, a 3.5 μl sample (prepared as described above) was placed on a plasma-cleaned (Fishione Inc.). Quantifoil holey carbon EM grid (SPI Supplies), blotted with filter paper, and flash-frozen in liquid ethane using a Leica EM GP (Leica Microsystems). The grids were subsequently stored in liquid nitrogen. The vitrified samples were imaged at liquid nitrogen temperature on a Tecnai 20 FEG electron microscope (FEI) operating at 200kV and images were collected with a 4 × 4 CCD camera.
Mattila J.P., Shnyrova A.V., Sundborger A.C., Hortelano E.R., Fuhrmans M., Neumann S., Müller M., Hinshaw J.E., Schmid S.L, & Frolov V.A. (2015). A hemi-fission intermediate links two mechanistically distinct stages of membrane fission. Nature, 524(7563), 109-113.
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2

Dynamin Interactions with Liposomes Visualized by Electron Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
For negative stain EM, samples (1-3 μM dynamin and 200 μM DOPS liposomes incubated in the presence or absence of 1 mM GTP or GMPPCP for 30 min at room temperature) were absorbed onto carbon-coated 400 mesh Cu/Rh grids (Ted Pella, Inc.), stained with 2% Uranyl Acetate, and imaged in a Tecnai 12 (FEI) transmission electron microscope at 120kV using a 2 × 2 Gatan CCD camera. For cryo-EM, a 3.5 μl sample (prepared as described above) was placed on a plasma-cleaned (Fishione Inc.). Quantifoil holey carbon EM grid (SPI Supplies), blotted with filter paper, and flash-frozen in liquid ethane using a Leica EM GP (Leica Microsystems). The grids were subsequently stored in liquid nitrogen. The vitrified samples were imaged at liquid nitrogen temperature on a Tecnai 20 FEG electron microscope (FEI) operating at 200kV and images were collected with a 4 × 4 CCD camera.
Mattila J.P., Shnyrova A.V., Sundborger A.C., Hortelano E.R., Fuhrmans M., Neumann S., Müller M., Hinshaw J.E., Schmid S.L, & Frolov V.A. (2015). A hemi-fission intermediate links two mechanistically distinct stages of membrane fission. Nature, 524(7563), 109-113.
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