Mesencult media

Manufactured by STEMCELL

Sourced in Canada

MesenCult media is a complete culture system designed for the growth and expansion of human mesenchymal stem cells (MSCs) in vitro. It provides a defined, serum-free, and xeno-free environment for the maintenance and differentiation of MSCs.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd45 magnetic beads, by Miltenyi Biotec (1 mentions) Crystal violet, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) α mem, by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

MesenCult basal media (3 citations) MesenCult Expansion Media (2 citations)

6 protocols using mesencult media

Isolation and Culture of Mouse Bone Marrow Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly harvested mouse BM cells were enriched for the CD45^neg fraction using anti-CD45 magnetic beads (Miltenyi Biotech), and 1×10⁶ CD45-negative cells were cultured in 6-well adherent tissue culture plates using MesenCult media (Stem Cell Technologies, Vancouver, BC) with 10% FBS. One half of the media was replaced after 7 days and on day 14 cells were stained with Giemsa staining solution (EMD Chemicals, Billerica, MA) and colonies enumerated. For in vitro BM stromal cell culture, CD45-negative BM cells were irradiated at 550 cGy, cultured in mouse MesenCult medium with or without SDF-1 (10 ng/ml) for 3 days, and treated with YM155 (10 ng/ml) or LY294002 (10 nM). To measure the phospho AKT, CD45-negative BM cells were cultured overnight in MesenCult medium, followed by 30 min treatment with SDF-1, and intracellular phospho AKT was measured by flow cytometry. For in vivo experiments, mice were irradiated at 650 cGy, and treated with SDF-1 (100 ug/kg/day) alone or in combination with YM155 (10 mg/kg/day) for 3 days.

Singh P., Mohammad K.S, & Pelus L.M. (2020). CXCR4 expression in the bone marrow microenvironment is required for hematopoietic stem and progenitor cell maintenance and early hematopoietic regeneration after myeloablation. Stem cells (Dayton, Ohio), 38(7), 849-859.

+ Open protocol

+ Expand

Rat Bone Marrow Mesenchymal Stem Cell Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The MSCs were obtained from three 6-month-old Sprague-Dawley female rats. After cervical dislocation, bone marrow heterogeneous cell population was obtained from femur and tibia by flushing DMEM (Dulbecco's modified Eagle's medium, Invitrogen, Paisley, UK) containing 10% FBS (fetal bovine serum, HyClone, Logan, UT, USA). The isolated MSCs were cultured in plastic culture dishes with MesenCult Media (StemCell Technology, Vancouver, Canada) including 20% supplement (StemCell Technology) and a 1% penicillin/streptomycin solution (HyClone). The cells were cultured in a 5% CO₂ incubator at 37°C. The medium of cells was changed twice a week for 14 days.

Akhan E., Tuncel D, & Akcali K.C. (2015). Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking. BioMed Research International, 2015, 298430.

+ Open protocol

+ Expand

Colony Formation Assay for BMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMSCs were plated into a six well culture plate (SPL) at 1000 cells/well density in Mesencult™ Media (Stem Cell Technologies) at 37 °C under 1% hypoxic conditions and humidity. Half of the medium was changed every three days. After two weeks of culture, the cells were fixed in 10% paraformaldehyde (Sigma) at room temperature for 30 min and stained with 1% crystal violet (Sigma) for 10 min at room temperature. The average number of colonies was counted.

Soh S., Han S., Ka H.I., Mun S.H., Kim W., Oh G, & Yang Y. (2023). Adiponectin affects the migration ability of bone marrow-derived mesenchymal stem cells via the regulation of hypoxia inducible factor 1α. Cell Communication and Signaling : CCS, 21, 158.

+ Open protocol

+ Expand

Isolation and Culture of Mouse BMMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The bone marrow cells were collected from the femurs and tibias of SAMP10 and SAMR1 mice, and the BMMSCs were then cultured as described in our previous report [9 (link)]. Briefly, the BMMSCs were cultured using MesenCult media according to the manufacturer’s instructions (Stem Cell Technology, Vancouver, BC, Canada). After passage, the cells were further cultured in fresh αMEM (Life Technologies Corporation, NY, USA), including 10% FCS and 1% antibiotic-antimycotic solution (Life Technologies). The 3rd passage cells were used for analysis.

Li M., Guo K., Adachi Y, & Ikehara S. (2016). Immune Dysfunction Associated with Abnormal Bone Marrow-Derived Mesenchymal Stroma Cells in Senescence Accelerated Mice. International Journal of Molecular Sciences, 17(2), 183.

+ Open protocol

+ Expand

Analyzing MSC-Macrophage Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

MSCs and EYFP+ macrophages were allowed to interact for 6 hours and washed with PBS. Using FACS sorting, CD90+ and CD90- cells were isolated, and doublet cells within CD90+ or CD90- cell populations were gated. In addition, EYFP+ macrophage populations within the CD90+/- doublet cells were also gated for further analysis.
Bone marrow from a young mouse (8 weeks) was cultured in a 10 cm plate with MesenCult media (StemCell Technologies) according to protocol. On day 3, bone marrow from a Lyzs-Cre-ROSA-EYFP mouse was cultured with L929 media for seven days using established protocol. On day 10, 3 million EYFP+ macrophages were added per each of the 10cm plates on top of the MSCs of the same animal. After 6 hours, the unbound cells were washed away with PBS, and the cells were stained with CD90 antibody for FCM.

Amini-Nik S., Abdullahi A., Vinaik R., Yao R.J., Yu N., Datu A., Belo C, & Jeschke M.G. (2022). Aging Impairs the Cellular Interplay between Myeloid Cells and Mesenchymal Cells during Skin Healing in Mice. Aging and Disease, 13(2), 540-551.

+ Open protocol

+ Expand

Preparation of hUCB-MSCs Secretome

Check if the same lab product or an alternative is used in the 5 most similar protocols

Preparation of hUCB-MSCs-derived secretome was conducted as described in a previous study. 12 HUCB-MSCs (3H Biomedical AB, Uppsala, Sweden) was cultured in Mesencult media (StemCell Technologies Inc., Vancouver, Canada) containing penicillin and streptomycin. Upon reaching 80% confluency, the media was replaced with supplement-free media and incubated for 24 hours. The media was collected and centrifuged. Supernatant was used as a conditioned medium contained with hUCB-MSCs-derived secretome.

Oktaviono Y.H., Susanti M., Lefi A., & Sandra F. (2020). Human Umbilical Cord Blood-derived Secretome Enhance Endothelial Progenitor Cells Migration on Hyperglycemic Conditions. Pharmacognosy Journal, 12(4), 793-797.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!