After thawing on wet ice, 5 μL IS working solution was added to 110.5 μL aliquots of calibration standards and QC samples spiked with acetic acid and ascorbic acid. Actual plasma samples for quantitative determination were required to be incubated with 200 units of each of sulfatase and β-glucuronidase for 30 min prior to the addition of IS. Thereafter, 1000 μL MTBE was added to extract both analytes. The samples were vortexed for 10 min and centrifuged at 15,000 rpm for 10 min at 4 °C. Then, 800 μL supernatant was transferred and evaporated to dryness under vacuum using an SPD1010 SpeedVac Concentrator (Thermo Fisher Scientific, Waltham, MA, USA) at 45 °C, 5.1 Vac for 2 h. The residue was reconstituted with 80 μL mobile phase. After centrifugation at 13,000 g for 10 min at 4 °C, 10 μL supernatant was injected for LC-MS/MS analyses.
Spd1010 speedvac concentrator
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SPD1010 SpeedVac Concentrator is a laboratory equipment designed for the concentration of liquid samples. It utilizes vacuum and temperature control to efficiently remove solvents from samples, leaving the desired components behind.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using spd1010 speedvac concentrator
1
Quantitative Determination of Analytes
2
Plasma Protein Precipitation Protocol
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All plasma samples including CS samples, QC samples, and plasma samples from pharmacokinetic study were prepared by protein precipitation. In addition, 100 μL plasma was spiked into 500 μL ACN, which contained 20 ng/mL IS. After vortex for 5 min and centrifugation at 16,000× g for 10 min at 4 °C, 480 μL of the supernatant was aspirated and evaporated using an SPD1010 SpeedVac Concentrator (Thermo Fisher Scientific, Waltham, MA, USA) at 45 °C, 5.1 Vac for 2.5 h. The residue was reconstituted in 80 μL of 15% ACN in water.
3
Quantitative Determination of Trandolapril and Trandolaprilat
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Trandolapril‐phenyl‐d5 and trandolaprilat‐phenyl‐d5 were respectively used as the internal standards (IS) for the quantifications of trandolapril and trandolaprilat. The calibration curves consisted of trandolapril and trandolaprilat at concentrations of 10, 30, 100, 300, 1000, and 3000 pg/ml. Quality control (QC) samples containing both analytes were prepared at concentrations of 30 pg/ml (low), 300 pg/ml (medium), and 1000 pg/ml (high). A solid‐phase extraction method was used to extract analytes from plasma samples. Briefly, 200 µL of plasma sample was mixed with 20 µl of IS working solution and 800 µl of 0.1% formic acid. The final concentrations of trandolapril‐phenyl‐d5 and trandolaprilat‐phenyl‐d5 were 200 pg/ml and 2 ng/ml, respectively. The mixture was vortexed at 1500 rpm for 2 min followed by solid‐phase extraction on Waters Oasis HLB columns according to the manufacturer’s instructions. The eluent was vacuum dried in a SpeedVac SPD1010 concentrator (Thermo Scientific) and reconstituted in 100 µl of 0.1% formic acid. Following centrifugation at 17,000 × g for 15 min, at 4°C, 4 µl of the supernatant was injected into an LC‐MS/MS system for the determination of trandolapril and trandolaprilat concentrations.
4
Quantitative Analysis of Plasma Drugs
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