The photomicrographs shown in Figures 4 -6 were produced by digital photography using a DS-Fi1 CCD color camera (2560 x 1920 pixels; Nikon) (Figures 4 and 5 ) or a CoolSNAP HQ2 CCD monochrome camera (1392 x 1040 pixels; Photometrics, Tucson, AZ, United States) (Figure 6 ) attached to an Eclipse Ti-E inverted microscope (Nikon) and NIS-Elements AR software (Nikon), using the following objectives (all from Nikon): PlanFluor 4 × (NA = 0.13), 10× (NA = 0.3) and 20× (NA = 0.45). The final figures were constructed using Corel Photo-Paint X7 and Corel Draw X7 (both versions 17.5.0.907; Corel, Ottawa, Canada). Only minor adjustments of contrast and brightness were made using Corel Photo-Paint, without altering the appearance of the original materials.
Ds fi1 ccd color camera
Manufactured by Nikon
Sourced in United States
The DS-Fi1 CCD color camera is a high-resolution digital imaging device designed for laboratory and scientific applications. It features a 5-megapixel CCD sensor, supporting a maximum resolution of 2560 x 1920 pixels. The camera is capable of capturing images and video at various frame rates, making it suitable for a wide range of microscopy and imaging tasks.
Automatically generated - may contain errors
Lab products found in correlation
Eclipse ti e inverted microscope, by Nikon (2 mentions)
Photo paint x7, by Corel (1 mentions)
Nis elements ar software, by Nikon (1 mentions)
Coreldraw x7, by Corel (1 mentions)
Plan apo 20 objective, by Nikon (1 mentions)
Lsm 710 axio observer confocal z1 laser scanning microscope, by Zeiss (1 mentions)
Anti α syn antibody, by BD (1 mentions)
Eclipse ti, by Nikon (1 mentions)
Eclipse 90i microscope, by Nikon (1 mentions)
4 protocols using ds fi1 ccd color camera
1
Photomicrography Protocol for Microscopy
2
Quantifying Microvascular Density in MI Border Zone
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The microvessel density was determined on up to four representative sections from each animal showing the left ventricular border zone of MI. Only sections that showed at least 30% of both scar tissue and viable myocardium were considered in this analysis. To this end, photomicrographs covering the entire section were taken with a DS-Fi1 CCD color camera (Nikon) attached to an Eclipse Ti-E inverted microscope (Nikon) using a PlanApo 20× objective (NA = 0.75) (Nikon). Images were analyzed using ImageJ software (U. S. National Institutes of Health, Bethesda, MD, United States). Using a defined grid of ten fields per section with an area of 0.3 mm² per field, two independent, blinded evaluators determined the number of microvessels per field (microvessels with a diameter between 2 and 10 µm were counted). Microvessel density was calculated based on microvessel counts on a total of 202 fields (animals in group 1) or 247 fields (animals in group 2), respectively.
3
Immunohistochemical Analysis of Parkinson's Disease
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Slides containing formalin fixed, paraffin embedded brain sections of advanced PD and controls, immunoreacted with anti SMI-32 ab [40 (link)] or anti α-Syn antibody (BD Transduction Labs), were provided by the Multiple Sclerosis Society Tissue Bank. Otherwise, slides were processed for immunostaining as previously described [39 (link)]. The antibodies used are listed in supplementary Table S2 . Images were acquired using a Nikon Ti Eclipse motorized inverted microscope with DIC, phase epi-fluorescence optics and Perfect Focus System (PFS). Equipped with a Nikon DS-Fi1 color CCD camera and NIS-Elements image acquisition software. Fluorescence images were acquired using a Zeiss LSM 710 Axio Observer confocal Z1 laser scanning microscope (as above). All images were taken using the same settings, and on the same day. The specific signal inside WMTs was quantified per area and normalized to the nonspecific signal outside of WMTs. Quantification of SMI-32, vGlut1 and TH immunoreactivity in the caudate were performed based on 6–10 fields per brain at × 20 magnification (Image J). Fields were chosen randomly. Images were taken and analyzed blindly to tissue classifying information.
4
Lung Metastasis Histological Analysis
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Left lung lobes were cryosectioned completely by taking 10 μm sections in 200 μm-intervals (9-13 sections/lung lobe). The sections were stained with hematoxylin/eosin (H&E; HistoLab Products AB, Göteborg, Sweden). Metastases were counted using a Nikon Eclipse 90i microscope and a 20X objective. Images were taken using a DS-Fi1 color CCD camera (Nikon Instruments Inc.).
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