Anti zo 1 21 773 1 ap

Manufactured by Proteintech

Sourced in China

Anti-ZO-1 (21,773-1-AP) is a laboratory research antibody product manufactured by Proteintech. The antibody is designed to detect the ZO-1 protein, which is a tight junction-associated protein. This product is intended for use in various research applications involving the identification and study of the ZO-1 protein.

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Ab129119, by Abcam (1 mentions) Anti flag 20543 1 ap, by Proteintech (1 mentions) Anti occludin 13409 1 ap, by Proteintech (1 mentions) Bs 0295g hrp, by Bioss Antibodies (1 mentions) Ab199529, by Abcam (1 mentions) Bs 0296g hrp, by Bioss Antibodies (1 mentions) Anti gapdh 10494 1 ap, by Proteintech (1 mentions) A0516, by Beyotime (1 mentions) Thunderbird next sybr qpcr mix, by Toyobo (1 mentions) Luminata crescendo western hrp substrate, by Merck Group (1 mentions) Bca protein assay kit, by Fujifilm (1 mentions) Alexa fluor 488 conjugated anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions) Anti β actin antibody, by Cell Signaling Technology (1 mentions) Polyvinylidene fluoride pvdf membrane, by Merck Group (1 mentions)

2 protocols using anti zo 1 21 773 1 ap

Antibody Validation for Western Blot and Immunofluorescence

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For Western blot or immunofluorescence analysis, the following antibodies were used: anti-ADAM10 (A10438; ABclonal, Wuhan, China), anti-E-cadherin full length (3195; Cell Signaling Technology, Danvers, MA, USA), anti-E-cadherin NTF (A3044, ABclonal), anti-claudin-1 (ab129119; Abcam, Cambridge, MA, USA), anti-occludin (13,409-1-AP; Proteintech, Wuhan, China), anti-ZO-1 (21,773-1-AP; Proteintech), anti-HtrA1 (ab199529; Abcam), anti-MMP7 (10,374-2-AP; Proteintech), anti-FLAG (20,543-1-AP; Proteintech), and anti-GAPDH (10,494-1-AP; Proteintech). Horseradish peroxidase (HRP)-labeled goat anti-rabbit (bs-0295 G-HRP; Bioss, Beijing, China), HRP-labeled goat anti-mouse (bs-0296 G-HRP; Bioss), and Cy3-conjugated goat anti-rabbit (A0516; Beyotime) secondary antibodies were used.

Cao Q., Wei W., Wang H., Wang Z., Lv Y., Dai M., Tan C., Chen H, & Wang X. (2021). Cleavage of E-cadherin by porcine respiratory bacterial pathogens facilitates airway epithelial barrier disruption and bacterial paracellular transmigration. Virulence, 12(1), 2296-2313.

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Immunohistochemical and Molecular Analysis of Tight Junction Proteins

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Rabbit polyclonal anti-ZO-1 (#21773-1-AP) and anti-Claudin-1 (#28674-1-AP) antibodies were purchased from Proteintech. The rabbit polyclonal anti-β-actin antibody (#4967) was purchased from Cell Signaling Technology. Alexa Fluor 488-conjugated anti-rabbit IgG antibody (#A11008) and ProLong Gold antifade regent with 4',6-diamidino-2-phenylindole (DAPI) were purchased from Thermo Fisher Scientific. Alcian Blue (AB) solution (pH2.5) and BCA protein assay kit were purchased from FUJIFILM Wako Pure Chemical Co. Periodic acid-Schiff (PAS) stain kit and NucleoSpin RNA kit were obtained from Muto Pure Chemicals Co. and Macherey-Nagel GmbH and Co. KG, respectively. Moloney murine leukemia virus (M-MLV) reverse transcriptase and oligo (dT)₁₈ primers were purchased from Invitrogen; Thermo Fisher Scientific. Thunderbird^™ Next SYBR^® qPCR mix was purchased from Toyobo (Osaka, Japan). Polyvinylidene fluoride (PVDF) membranes and Luminata Crescendo Western HRP substrate were purchased from Merck Millipore. Enzyme-linked immunosorbent assay (ELISA) kit for IL-10 was obtained from LSBio.

Kawaguchi S., Sakuraba H., Kikuchi H., Matsuki K., Hayashi Y., Ding J., Tanaka Y., Seya K., Matsumiya T., Hiraga H., Fukuda S., Sasaki K, & Imaizumi T. (2023). Polygonum tinctorium leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice. Experimental and Therapeutic Medicine, 25(3), 112.

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