Speedvac high capacity concentrator

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SpeedVac High Capacity Concentrator is a laboratory equipment designed to gently remove solvents and concentrate samples. It utilizes a vacuum system and controlled temperature to efficiently evaporate liquid samples, preserving the integrity of the sample materials.

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Lab products found in correlation

Lichroprep si 60, by Merck Group (2 mentions) Ultrasonic cleaner, by Emerson (1 mentions) Prominence column oven, by Shimadzu (1 mentions) Cto 20a column oven, by Shimadzu (1 mentions) Refrigerated vapor trap, by Thermo Fisher Scientific (1 mentions)

3 protocols using speedvac high capacity concentrator

HPLC-MS/MS Analysis of Samples

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A Thermo Scientific Savant Speedvac high capacity concentrator in combination with a Thermo Scientific Savant Refrigerated Vapor Trap was used for evaporation under vacuum of the samples during sample pretreatment. An ultrasonic cleaner from Branson was used for reconstitution and homogenization of the residue.
The HPLC-MS/MS system consisted of an Ultimate 3000 autosampler and pump with inline degasser, all of Dionex (Sunnyvale, CA, USA), connected to a degasser from LC Packings. The autosampler with a 100 μL sample loop was coupled to an AB Sciex API4000 mass spectrometer (Framingham, MA, USA) via a Shimadzu Prominence Column Oven (type CTO-20A) which was kept at 35 °C. Separation was performed with an Agilent column 2.1 × 100mm packed with material of Zorbax Extend 3.5 μm C-18 and kept at 35 °C using a CTO-20A column oven (Shimadzu, Kyoto, Japan). Instrument control and data acquisition was done using Analyst v1.6.2 and DCMS-link v2.12 software (Dionex).

Kroon M.A., van Laarhoven H.W., Swart E.L., Kemper E.M, & van Tellingen O. (2023). A validated HPLC-MS/MS method for simultaneously analyzing curcumin, demethoxycurcumin, bisdemethoxycurcumin, tetra-hydrocurcumin and piperine in human plasma, urine or feces. Heliyon, 9(5), e15540.

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Oxytocin Radioimmunoassay Protocol

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Saliva samples were assayed by RIAgnosis according to a previously described procedure 17. Briefly, samples were extracted using LiChroprep Si60 (Merck, Hesse, Germany) which was heat‐activated at 700°C for three hours. After evaporation using a SpeedVac High Capacity Concentrator (Thermo Scientific, Waltham, MA, USA), 50 μL of assay buffer was added. The oxytocin was then measured with a highly sensitive and specific radioimmunoassay, standardised and validated in human studies. The detection limit was in the 0.5 pg/sample range, the intra‐assay variation was <8%, and the <0.7% cross‐reactivities were considered negligibly small 18.

Kommers D., Broeren M., Andriessen P., Oei S., Feijs L, & Bambang Oetomo S. (2016). Pilot study demonstrates that salivary oxytocin can be measured unobtrusively in preterm infants. Acta Paediatrica (Oslo, Norway : 1992), 106(1), 34-42.

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Saliva Oxytocin Extraction and Measurement

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After finishing a saliva collection, nurses immediately placed the cotton swabs in tubes and transferred them to the laboratory for storage at -20 °C. When all samples were collected, the tubes were allowed to reach room temperature for 30 min. Subsequently, they were centrifuged at 4 °C 3000 r/min for five minutes, after which the saliva was pooled into vials. We immediately refroze the filled vials at -20 °C in order to ship them to RIAgnosis (Munich, Germany) according to their protocols. There, saliva was analyzed according to the previously described procedure (Kagerbauer et al., 2013; (link)Kommers, Broeren et al., 2017) (link). Briefly, samples were extracted using LiChroprep Si60 (Merck, Hesse, Germany) which was heat-activated at 700 °C for three hours.
After evaporation using a SpeedVac High Capacity Concentrator (Thermo Scientific, Massachusetts, USA) 50 μl of assay buffer was added. The oxytocin was then measured with a highly sensitive and specific radio-immuno assay, standardized and validated in human studies. The detection limit was in the 0.5 pg/sample range, the intraassay variation was less than 8% and the < 0.7% cross reactivity was considered negligibly small.

Kommers D., Broeren M., Oei G., Feijs L., Andriessen P, & Bambang Oetomo S. (2018). Oxytocin levels in the saliva of preterm infant twins during Kangaroo care. Biological psychology, 137.

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