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Modified two photon microscope

Manufactured by Bruker
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The modified two-photon microscope is a specialized imaging instrument designed for high-resolution, three-dimensional visualization of biological samples. It utilizes the principles of two-photon excitation to achieve deep tissue penetration and enhanced optical sectioning capabilities.

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Lab products found in correlation

Prairieview software, by Bruker (2 mentions) 1.0 na water immersion objective, by Olympus (2 mentions)

2 protocols using modified two photon microscope

1

Two-Photon Calcium Imaging of Neural Networks

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Calcium imaging experiments were performed using a modified two-photon microscope (Bruker) outfitted with a 25× 1.0NA water immersion objective (Olympus) and a mode locked Ti:sapphire laser (Verdi 18W, Coherent) at 940nm. A custom-made computerized, motorized goniometer was used to subtly and reproducibly angle the head so that the cranial window was orthogonal to the beam path. Images were acquired using Prairie View software (Bruker) at 64Hz, and every 4 images were averaged, yielding an effective sampling rate of 16Hz. Data was acquired from an area approximately 430μm × 430μm with 256 × 256 pixels. Multiple non-overlapping field of view were imaged from each mouse over ~7 days. Following injections of EnVA-N2cΔG-tdTomato, fields of view from functional imaging sessions were identified by first aligning surface vasculature, then carefully aligning basal GCaMP fluorescence signals to reference images taken during functional imaging. Z stacks and 2D images of tdTomato fluorescence were acquired at a wavelength of 1040nm.
Nelson A., Abdelmesih B, & Costa R.M. (2021). Corticospinal populations broadcast complex motor signals to coordinated spinal and striatal circuits. Nature neuroscience, 24(12), 1721-1732.
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2

Two-Photon Calcium Imaging of Neural Networks

Check if the same lab product or an alternative is used in the 5 most similar protocols
Calcium imaging experiments were performed using a modified two-photon microscope (Bruker) outfitted with a 25× 1.0NA water immersion objective (Olympus) and a mode locked Ti:sapphire laser (Verdi 18W, Coherent) at 940nm. A custom-made computerized, motorized goniometer was used to subtly and reproducibly angle the head so that the cranial window was orthogonal to the beam path. Images were acquired using Prairie View software (Bruker) at 64Hz, and every 4 images were averaged, yielding an effective sampling rate of 16Hz. Data was acquired from an area approximately 430μm × 430μm with 256 × 256 pixels. Multiple non-overlapping field of view were imaged from each mouse over ~7 days. Following injections of EnVA-N2cΔG-tdTomato, fields of view from functional imaging sessions were identified by first aligning surface vasculature, then carefully aligning basal GCaMP fluorescence signals to reference images taken during functional imaging. Z stacks and 2D images of tdTomato fluorescence were acquired at a wavelength of 1040nm.
Nelson A., Abdelmesih B, & Costa R.M. (2021). Corticospinal populations broadcast complex motor signals to coordinated spinal and striatal circuits. Nature neuroscience, 24(12), 1721-1732.
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