Perm wash buffer 554723

Manufactured by BD

Sourced in United States

Perm/Wash buffer (554723) is a laboratory reagent used in various cell analysis and processing applications. It is designed to maintain cell structure and function during permeabilization and washing steps. The buffer composition is formulated to preserve the integrity of cellular components while facilitating access to intracellular targets for further analysis.

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Lab products found in correlation

Monensin, by Merck Group (2 mentions) 2 4 dinitrofluorobenzene dnfb 2 4 dinitrobenzenesulphonic acid dnbs trypsin from bovine pancreas, by Merck Group (2 mentions) Recombinant murine il 2 i0523, by Merck Group (2 mentions) Anti human nkg2d 1d11, by BioLegend (2 mentions) Anti human cd56 hcd56, by BioLegend (2 mentions) Mouse igg2b isotype control 133303, by R&D Systems (2 mentions) Stempro accutase a11105 01, by Thermo Fisher Scientific (2 mentions) Efluor 780, by Thermo Fisher Scientific (2 mentions) Ionomycin, by Merck Group (2 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (2 mentions) Rabbit anti γh2ax, by Cell Signaling Technology (1 mentions) Rabbit anti nox4, by Proteintech (1 mentions) Fitc conjugated goat anti rabbit antibody, by Abcam (1 mentions) Cytoperm permeabilization buffer plus, by BD (1 mentions) Methocult gf m3534 medium, by STEMCELL (1 mentions) 2 7 dichlorodihydrofluorescein diacetate dcf da, by Merck Group (1 mentions) Sitagliptin, by Merck Group (1 mentions)

Close spelling variants of this product

Perm/Wash buffer Perm/Wash Perm buffer Wash buffer

3 protocols using perm wash buffer 554723

Quantifying Immune Cell Activation

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2,4-dinitrofluorobenzene (DNFB), 2,4-dinitrobenzenesulphonic acid (DNBS), trypsin from bovine pancreas, phorbol 12-myristate 13-acetate (PMA), ionomycin, monensin and recombinant murine IL-2 (I0523) were purchased from Sigma Aldrich (Brøndby, Denmark). Anti-mouse-NKG2D (C7), anti-mouse-NKG2D (CX5) and fixable viability dye eFluor780 were purchased from eBioscience (San Diego, CA, USA). Anti-human-CD8 (RPA-T8), anti-human-CD4 (OKT4), anti-human-CD3 (OKT3), anti-human-NKG2D (1D11) and anti-human-CD56 (HCD56) were purchased from Bio-Legend (San Diego, CA, USA). Anti-mouse-CD16/CD32 (2.4G2), anti-mouse-γδ TCR (GL3), anti-mouse-Vγ3 (536), anti-mouse-CD3ε (145-2C11), anti-mouse-IFN-γ (XMG1.2), anti-human γδ TCR (B1.1), anti-human/mouse CLA (HECA-452), streptavidin-APC-Cy7 (554063), Cytofix/Cytoperm (554722) and Perm/Wash buffer (554723) were all purchased from BD Biosciences (Albertslund, DK). Anti-mouse-Rae-1 (237104), anti-mouse-H60 (205326), anti-mouse-Mult-1 (186107), antimouse-NKG2D (191004), anti-human-MICA (159227), anti-human-MICB (236511) and mouse IgG2B isotype control (133303) were purchased from R&D Systems (Abingdon, UK). StemPro Accutase (A11105-01) was purchased from Life Technologies (Nærum, DK).

Nielsen M.M., Dyring-Andersen B., Schmidt J.D., Witherden D., Lovato P., Woetmann A., Ødum N., Poulsen S.S., Havran W.L., Geisler C, & Bonefeld C.M. (2015). NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. The Journal of investigative dermatology, 135(5), 1311-1319.

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Quantifying Immune Cell Activation

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Multiparametric Flow Cytometry Analysis

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Biotin conjugated anti-mouse-CD4 (clone 34 GK1.5), anti-mouse-CD8 (clone 53-6.7), anti-mouse-CD11b (clone M1/70), anti-mouse-CD45R/B220 (clone RA3-6B2), anti-mouse-Ly6G/Gr-1 (clone RB68C5), anti-mouse-Ter119 (clone Ter119), anti-mouse-CD117 (c-kit)-APC (clone 2B8), anti-mouse -Ly-6A/EA (Sca-1)-PE (clone D7), and PERCP-conjugated streptavidin were purchased from eBioscience (San Diego, CA, USA). In addition, 2,7-dichlorodihydrofluorescein diacetate (DCFDA) was purchased from Sigma (St. Louis, MO, USA). MethoCult GF M3534 medium was purchased from Stem Cell Technologies (Vancouver, Canada). MitSox red mitochondrial superoxide indicator was obtained from Life Technologies (Grand Island, NY, USA). Rabbit anti-γH2AX was obtained from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-NOX4 was obtained from Proteintech (Wuhan, China). FITC-conjugated goat anti-rabbit antibodies were obtained from Abcam Biotechnology (Cambridge, MA, USA). Cytofix/Cytoperm buffer (554722), Perm/Wash buffer (554723), and Cytoperm Permeabilization Buffer Plus (561651) were obtained from BD Pharmingen (San Diego, CA, USA). Sitagliptin was obtained from Merck Sharp & Dohme (South Granville, NSW, Australia).

Wang M., Dong Y., Wu J., Li H., Zhang J., Lu L., Zhang Y., Zhou Z., Fan S., Li D, & Meng A. (2020). Sitagliptin Mitigates Total Body Irradiation-Induced Hematopoietic Injury in Mice. Oxidative Medicine and Cellular Longevity, 2020, 8308616.

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