Ampliprep taqman hiv 1 v2.0 system

HIV-1 samples were cultured from peripheral blood monoclonal cells (PBMCs). PBMCs were isolated from HIV-1 using the Ficoll-Hypaque density gradient cell configuration. The obtained sample was stimulated by phytohemagglutinin (PHA) for a 3 day period and co-cultured with HIV-1 positive PBMCs. Then, samples were incubated at 37 °C in the presence of 5% of CO₂. P24 titer measurement was carried out on the supernatant of the co-culture sample with ELISA (Perkin Elmer, NEK050b). When p24 in the sample reached the level of 20 ng ml⁻¹, the co-culture process was stopped. The stock concentration of the sample was 10⁸ copies per ml. The viral load test was performed by using a Roche-COBAS AmpliPrep TaqMan HIV-1 v2.0 system which was located in the microbiology laboratory at Brigham and Women’s Hospital (BWH).^{4 (link)}