Blood sample were collected from the angular vein with EDTA for lipid analysis. Serum TC, TG, HDL-C, and LDL-C were quantified on a Hitachi 7600-020 analyzer by a specialist who was blinded to this study, as previously described [22 (link)]. Plasma concentration of MCP-1 (ab100721, Abcam) and VCAM-1 (ab201218, Abcam) was determined using an ELISA kit, following the manufacturer's instructions.
7600 020 analyzer
Manufactured by Hitachi
Sourced in Japan
The 7600-020 analyzer is a laboratory equipment piece designed for analytical tasks. It features advanced capabilities for accurate measurements and data processing. The core function of this analyzer is to provide reliable and precise analysis results, supporting various scientific and industrial applications.
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4 protocols using 7600 020 analyzer
1
Lipid Profiling and Inflammatory Markers
2
Retrospective Analysis of Muscle Disorders
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The clinical data (including the chief complaint, history of present illness, growth and development history, past medical history and family history) of all subjects were collected and reviewed retrospectively. Blood samples were collected and levels of serum enzymes CK, liver function and cardiac enzymes concentrations were measured using a Hitachi 7600-020 analyzer (Hitachi, Ltd., Tokyo, Japan). All assays were conducted according to the manufacturer's instructions using the same batch reagent. Echocardiography findings and muscle biopsies were analyzed.
3
Serum AFP and SOD Analysis
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The participants were fasted overnight before blood collections. The participants sat for at least 15 min before blood was collected. A volume of 6 mL of venous whole blood was collected from each subject and placed into serum gel tubes (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA). Samples were allowed to stand for 20 min at 4°C, centrifuged at 2500 g for 20 min at 4°C (Anhui USTC Zonkia Scientific Instrument Co., Ltd, Anhui, China) and stored at 4°C until analysis.
Serum AFP levels were determined by an electrochemiluminescence immunoassay using a Cobas® 6000 system E601 (Elecsys module) immunoassay analyzer (Roche Diagnostics, GmbH, Mannheim, Germany). SOD concentrations were measured by colorimetry with a Hitachi 7600-020 analyzer (Hitachi Corp, Tokyo, Japan). All assays were conducted according to the manufacturer’s instructions by the same operator using the same batch reagent.
Serum AFP levels were determined by an electrochemiluminescence immunoassay using a Cobas® 6000 system E601 (Elecsys module) immunoassay analyzer (Roche Diagnostics, GmbH, Mannheim, Germany). SOD concentrations were measured by colorimetry with a Hitachi 7600-020 analyzer (Hitachi Corp, Tokyo, Japan). All assays were conducted according to the manufacturer’s instructions by the same operator using the same batch reagent.
4
Comprehensive Liver Evaluation Protocol
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Blood samples were obtained from all patients on the day before liver biopsy. Serum markers were measured in either fresh blood or frozen serum samples stored at − 40 °C. Hematological (Sysmex XE-2100, Sysmex Corporation, Japan) or common biochemical (Hitachi 7600-020 Analyzer, Hitachi, Japan; Wako Diagnostics reagents, Wako Pure Chemical Industries Ltd, Japan) and coagulation function (MC-2000 blood coagulation analyzer, Meichuang Company, Germany) tests were performed using standard methodologies. The reference value was 5–50 IU/L for alanine aminotransferase (ALT) (IFCC, 37 °C), 15–60 IU/L for GGT, 40–55 g/L for ALB, and 9–13 s for prothrombin time (PT). Hepatitis virus markers (Abbott ARCHITECT i2000 SR system, Abbott Laboratories, Abbott Park, IL, USA) including HBsAg, hepatitis B surface antibody (HBsAb), hepatitis B early antigen (HBeAg), hepatitis B early antibody (HBeAb), hepatitis B core antibody (HBcAb), and anti-HCV were measured with Clinical Laboratory Improvement Amendment (CLIA) systems. HBV DNA concentrations were measured using the COBAS TaqMan assay (Roche Molecular Systems, Branchburg, NJ, USA), which has a lower limit of quantification of 100 copies/mL. All markers described above were measured by the Department of Laboratory Medicine, Shanghai First People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
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