Caspase 8 1c12 mouse mab

Cladribine (Sigma Co., St. Louis, MO) and entinostat (LC Laboratories, Inc., Woburn, MA) were dissolved in dimethyl sulfoxide (DMSO) to make a stock solution at 250 mmol/L and 200 mmol/L, respectively. The stock solutions were stored at −20°C.
The sources of antibodies for western blot assays were as follows: caspase-3 rabbit mAb (8G10), caspase-8 (1C12) mouse mAb, caspase-9 (Asp353) rabbit mAb, PARP rabbit mAb, P-Histone H2A.X (Ser139) rabbit antibody, Acetyl-Histone H3 (Lys9), Histone H3, P-CHK1 (Ser345) (133D3) rabbit mAb, CHK1 rabbit antibody, P-CHK2 (Thr68) rabbit polyclonal antibody, CHK2 rabbit polyclonal antibody and p21^Waf1/Cip1 (12D1) rabbit mAb (Cell Signaling Technology, Inc., Beverly, MA); Cyclin D1 rabbit mAb, E2F-1 mouse mAb (KH95), p27 (F-8) mouse mAb (Santa Cruz Biotechnology Inc., Santa Cruz, CA); β-actin mouse mAb (clone AC-75) (Sigma Co.). All other reagents were purchased from Sigma Co. unless otherwise specified.

SBC-3 cells (cell concentration: 5
× 10⁵ cells/mL) were cultured in a six-well flat-bottom
plate and treated with EtOH/H₂O (1:1), 10 μM cisplatin,
or 15 μM 1 for 24 h. The Western blotting analysis
was carried out by the same procedures as previously reported.^{26 (link)} The following antibodies were recruited: β-actin
(8H10D10 Mouse mAb, product number 3700, 1:1000; Cell Signaling Technology,
Danvers, MA, USA), caspase-8 (1C12 Mouse mAb, product number 9746,
1:1000; Cell Signaling Technology), caspase-9 (C9 Mouse mAb, product
number 9508, 1:2000; Cell Signaling Technology), caspase-3 (3G2 Mouse
mAb, product number 9668, 1:1000, Cell Signaling Technology), PARP
(46D11 Rabbit mAb, product number 9532, 1:1000; Cell Signaling Technology),
Bax (2D2 Mouse mAb, product number 89477, 1:1000; Cell Signaling Technology),
Bcl-2 (124 Mouse mAb, product number 15071, 1:1000; Cell Signaling
Technology), anti-rabbit IgG, horseradish peroxidase (HRP)-linked
antibody (product number 7074, 1:10000; Cell Signaling Technology),
and anti-mouse IgG, HRP-linked antibody (product number 7076, 1:10000;
Cell Signaling Technology). The signals were detected using ECL Prime
Western Blotting Detection Reagents (GE Healthcare, Boston, MA, USA),
and photographed by a LAS-3000 luminescent image analyzer (FUJIFILM,
Tokyo, Japan).