Mouse anti cytokeratin 18

HHV-6 antigen expression was analyzed by immunofluorescence with a mouse monoclonal antibodies (mAb) that recognized the glycoprotein gp116 (late antigen) of HHV-6 A and B (ABI, Columbia, MD, United States), as previously described (Marci et al., 2016 (link)). Epithelial and stromal cells were respectively stained with mouse anti-Cytokeratin-18, a heterotetramer cytoskeleton protein (CK18-FITC) and rabbit anti-vimentin-PE moAbs (Abcam, Cambridge, United Kingdom), respectively. eNK cells were stained for CD56-PE (BD, Milan, Italy). Epithelial cells were stained for IP-10-FITC (Interferon gamma-induced protein 10/CXCL10), Eotaxin-3-PE (CCL26) and MCP-1-PE (Monocyte chemo-attractant protein 1/CCL2) (eBiosciences, Waltham, MA, United States).

Immunofluorescence for HHV-6 antigen expression was performed with a mouse monoclonal antibodies (mAb) directed against p41 and IE2 (early antigen) and glycoprotein gp116 (late antigen) of HHV-6 A and B (ABI, Columbia, MD, USA), as previously described [30 (link)]. Epithelial and stromal cells were respectively stained with mouse anti-Cytokeratin- 18, a heterotetramer cytoskeleton protein (CK18) and rabbit anti-vimentin, an intermediate filament of the cytoskeleton proteins moAbs (Abcam, Cambridge, UK), respectively. Nuclei were counterstained with DAPI (4,6-diamidino-2-phenylindole-dihydrochroride) (Sigma-Aldrich, S.Louis, MO, USA).