Commercially available primary antibodies used in this study for immunoprecipitation (IP), immunofluorescence (IF), proximity ligation assay (PLA), Western blot (WB), and Simple Western capillary system (WES) were as follows: anti-GFP (abcam, ab6556, IF & WB 1:5000, PLA 1:2000), anti-NFH (abcam, ab72996, 1:2000), anti-Actin (MP, clone C4, 08691001, WB 1:5000), anti-GAPDH (Santa Cruz Biotechnology, sc-32233, WB 1:5000), anti-ERK1/2 (Sigma, M5670, WB 1:30000), anti-β-III tubulin TUJ1 (abcam, ab18207, IF 1:1000, WB: 1:6000), anti-Dynein heavy chain (DYNC1H1, Proteintech, #12345-1-AP, PLA 1:50), anti-importin beta 1 (MBS713065, rabbit, polyclonal, 1:1000), and rabbit anti-nucleolin (ProteinTech, 10556-1-AP, 1:100 for IF).
Sc 32233
Manufactured by Merck Group
Sc-32233 is a laboratory equipment product. It is a precision instrument designed for specific scientific applications. The core function of Sc-32233 is to provide accurate and reliable measurements for research and analytical purposes. Further details on its intended use are not available.
Automatically generated - may contain errors
2 protocols using sc 32233
1
Antibodies for Protein Localization and Expression
2
Antibody Validation for Cellular Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercially available primary antibodies (ABs) used in this study for immunoprecipitation (IP), immunofluorescence (IF), proximity ligation assay (PLA), Western blot (WB) and Simple Western capillary system (WES) were: Anti-GFP (abcam, ab6556, IF & WB 1:5000, PLA 1:2000), anti-NFH (abcam, ab72996, 1:2000), anti-Actin (MP, clone C4, 08691001, WB 1:5000), anti-GAPDH (Santa Cruz Biotechnology, sc-32233, WB 1:5000), anti-ERK1/2 (Sigma, M5670, WB 1:30000), anti-β-III tubulin TUJ1 (abcam, ab18207, IF 1:1000, WB: 1:6000), anti-Dynein heavy chain (DYNC1H1, Proteintech , #12345-1-AP, PLA 1:50), antiimportin beta 1 (MBS713065, rabbit, polyclonal, 1:1000).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!