HL60 (AML), K562 (CML) were cultured in in RPMI 1640 supplemented with 10% FCS and maintained at 37°C with 5% CO2. Molt-4, Jurkat, HSB-2 and TALL-1 (T-ALL) were cultured in RPMI 1640 supplemented with 20% FCS (DSMZ, Braunschweig, Germany). SUP-B15 (BCP-ALL) was cultured in McCoy’s 5A supplemented with 20% FCS (DSMZ, Braunschweig, Germany).
Rpmi1640
Manufactured by Leibniz Institute DSMZ
Sourced in Germany
RPMI1640 is a cell culture medium used for the in vitro cultivation of a variety of mammalian cells, including human cells. It is a complex formulation that provides the necessary nutrients, vitamins, and salts to support cell growth and proliferation. The core function of RPMI1640 is to maintain the optimal physiological conditions for cell culture, enabling researchers to study and manipulate cells in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Mccoy s 5a, by Leibniz Institute DSMZ (1 mentions)
Sup b15, by Leibniz Institute DSMZ (1 mentions)
Roswell park memorial institute (rpmi), by Welgene (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
Blasticidin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Leibniz Institute DSMZ (1 mentions)
Non essential amino acids (neaa), by Harvard Bioscience (1 mentions)
L glutamine, by Harvard Bioscience (1 mentions)
Rpmi 1640, by Harvard Bioscience (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Harvard Bioscience (1 mentions)
Penicillin streptomycin, by Harvard Bioscience (1 mentions)
Streptomycin, by PromoCell (1 mentions)
Eol 1, by Leibniz Institute DSMZ (1 mentions)
Penicillin, by Leibniz Institute DSMZ (1 mentions)
Streptomycin, by Leibniz Institute DSMZ (1 mentions)
Penicillin, by PromoCell (1 mentions)
4 protocols using rpmi1640
1
Culturing Leukemia Cell Lines
2
Cell Culture Practices: Maintaining Diverse Cell Lines
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KM12(Luc) was purchased from the JCRB cell bank. KM12 (Luc) was used within 6 months after purchase and the DNA profiles were confirmed using a short tandem repeat (STR) analysis by the Korean Cell Line Bank. KM12(Luc) cells were maintained in DMEM supplemented with 10% FBS and antibiotics. Parental Ba/F3 cells were purchased from DSMZ and were grown in RPMI1640 supplemented with 10% FBS and antibiotics in the presence of IL3, Transformed Ba/F3 cell lines were grown in the same media without IL3. Cells used in this study were negative for mycoplasma contamination. AN3-CA, HEC1A, U138, 8505C, MKN28, MKN45, A172, A375, Capan-1 HeLa, HEP3B, MCF-7 and MIA-paca-2 cells were maintained in DMEM (Welgene, Korea) supplemented with 10% FBS and antibiotics (Welgene, Korea). Otherwise, all cells were maintained in RPMI (Welgene, Korea) supplemented with 10% FBS and antibiotics (Welgene, Korea).
3
Cell Culture Protocol for Tumor and Transduced Cell Lines
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Tumor cell lines, U-251 MG and EGFRvIII-expressing U-251 MG (U-251 MG/EGFRvIII), were described previously (16) . Both cell lines were cultured in DMEM (Gibco) containing 10% FBS (Invitrogen) and 100 U/mL penicillin/streptomycin (Gibco). Tumor cell lines, U-87 MG/EGFRvIII and GOS-3/EGFRvIII, were generated by transduction of U-87 MG tumor cells (ATCC) and GOS-3 [German Collection of Microorganisms and Cell Culture (DSMZ)] with human EGFRvIII cDNA, and were cultured in RPMI1640 (Biochrom) and DMEM containing 10% FBS, 100 U/mL penicillin/streptomycin, and 5 mg/mL Blasticidin (Gibco). The tumor cell line, DK-MG (DSMZ), was cultured in RPMI1640 containing 10% FBS, 100 U/mL penicillin/ streptomycin, and 1 Â nonessential amino acids (NEAA, Biochrom). CHO cells were transduced with wild-type EGFR (HER1), HER2, HER3, HER4, and human and cynomolgus monkey EGFRvIII. CHO cells were cultured in DMEM containing 10% FBS, 10 mmol/L sodium hypoxanthine, 1.6 mmol/L thymidine (1 Â HT Supplement, Gibco), and 1 Â NEAA (Biochrom). Transduced CHO cells were cultured in DMEM containing 10% FBS, 2 mmol/L L-Glutamine (Biochrom), 100 U/mL penicillin/streptomycin, and 1 Â NEAA. T-cell lines, HPB-ALL (DSMZ) and LnPx4119 (described previously; ref. 15) , were cultured in RPMI1640 containing 10% FBS and 100 U/mL penicillin/ streptomycin. All cells were cultured at 37 C in a 5% CO 2 chamber.
4
Cell Culture Maintenance and Characterization
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The human colorectal cancer cell line HT29 (purchased from the European Cell Culture Collection) and the human chronic eosinophilic leukemia cell line EOL-1 (from DSMZ) were maintained in RPMI-1640 supplemented with 2 mM L-glutamine, 10% fetal calf serum (FCS), 100 μg penicillin/mL and 100 μg streptomycin/mL (latter reagents were purchased from PAA) at 37°C in a humidified atmosphere of 5% CO2. For all assays, HT29 cells were cultivated until 80% confluency; EOL-1 cells were grown in suspension. Primary human pulmonary microvascular endothelial cells (HPMECs) were obtained from Promocell, cultured in endothelial cell growth medium MV supplemented with the corresponding supplement mix as provided by the supplier, and 100 μg penicillin/mL and 100 μg streptomycin/mL. HPMECs were sub-cultured using a specific detach kit (PromoCell) according to the manufacturer’s instructions. All experiments with primary cells were performed during the first six passages.
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