For in vivo biodistribution studies, 4 × 105 Lin− BM cells from male and female P3D2F1 mice transduced with PGK-coRPK-WPRE LV were intravenously administered into lethally irradiated B6D2F1 congenic recipients of both sexes. Results were compared with a control group that received non-transduced cells (n = 6 male, and n = 2 females). 1 month after BM transplantation (see Table S1 ), mice were sacrificed by anesthetic overdose with Avertin (15 μL of a 2.5% solution/1 g weight). Animals were perfused with PBS/EDTA solution through a cardiac cannula and hematopoietic (PB, BM, spleen, thymus, LNs), and non-hematopoietic (liver, lung, kidney, pancreas, large and small intestine, stomach, gonads, brain, muscle, heart) organs were extracted and processed for histology, flow cytometry, analysis of VCN, and serology for the presence of HIV p24 protein, measured according to the manufacturer’s instructions (Alliance HIV-1 P24 Antigen ELISA Kit [96 Test] [NEK050001KT]; PerkinElmer) (see Supplemental text ).
Alliance hiv 1 p24 antigen elisa kit 96 test
Manufactured by PerkinElmer
The Alliance HIV-1 P24 Antigen ELISA Kit [96 Test] is a laboratory equipment product designed for the detection and quantification of the HIV-1 p24 antigen in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to provide a reliable and standardized method for measuring the presence of the HIV-1 p24 antigen.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alliance hiv 1 p24 antigen elisa kit 96 test
1
In Vivo Biodistribution of Transduced BM Cells
2
Vaginal HIV-1 Challenge in Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vaginal drug administration was carried out as described previously25 ,27 (link) and summarized in Fig. 1 . The employed drug concentrations in the single- and multiple drug dosing groups are provided in Supplementary Data S1 –S4 . Stocks of HIV-1 JR-CSF were prepared as previously described28 (link),37 (link) and standardized by p24 ELISA using the Alliance HIV-1 P24 ANTIGEN ELISA Kit (96 Test) (Perkin Elmer, Waltham, MA), according to the manufacturer’s instructions. Prior to inoculation, mice were anesthetized with isoflurane. Aliquots (5 μL) of drug solutions in PBS were applied vaginally through a pipet tip. The rear half of the mouse remained elevated during the procedure to reduce chance of back-flow from the vaginal cavity during the recovery. Within 30 min (typically 15–25 min) post-drug application, mice were vaginally challenged with HIV-1 (5 µL, corresponding to 200 ng of p24). This inoculum is a standard high viral load for successful vaginal infection (1 ng of p24 corresponds to ca. 10 infectious units). Methods used for the atraumatic vaginal HIV-1 challenge are described elsewhere38 (link),41 (link)–44 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!