Log In Sign up
The largest database of trusted experimental protocols

Hplc grade acetone

Manufactured by Lach-Ner
Visit Supplier

HPLC grade acetone is a high-purity solvent used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic odor. HPLC grade acetone meets the stringent purity requirements for use in HPLC analysis and other sensitive analytical techniques.

Automatically generated - may contain errors

Lab products found in correlation

Hplc water, by Merck Group (2 mentions) Thermo ultimate 3000, by AB Sciex (2 mentions) Hplc formic acid, by Merck Group (2 mentions) Tripletof 5600 mass spectrometer, by AB Sciex (2 mentions)

2 protocols using hplc grade acetone

1

Quantification of CuET in HPLC-ESI-QTOF

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HR-MRM analysis was performed on HPLC-ESI-QTOF system consisting of HPLC chromatograph Thermo UltiMate 3000 with AB Sciex TripleTOF 5600+ mass spectrometer, using the DuoSpray ESI source operated at ion source voltage 5500 V, ion source gas flow rates 40 units, curtain gas flow rate 30 units, declustering potential 100 V and temperature 400°C. Data were acquired in Product ion mode with two parent masses 358.9 and 360.9 for analysis of CuET. Chromatographic separation was done by PTFE column especially designed for analysis of strong metal chelators filled by C18 sorbent (IntellMed, cat.no.IM_301). Analysis was performed at room temperature and flow rate 1500 μL/min with isocratic chromatography. Mobile phase consisted of HPLC grade acetone (Lachner) 99.9%, HPLC water (Merck Millipore) 0.1% and 0.03% HPLC formic acid (Sigma). Acquired mass spectra were evaluated in software PeakView 1.2, where extracted ion chromatograms of transitions 88.0 and 116.0 (common for both parent masses) with 0.1 mass tolerance was Gaussian smoothened with width of 2 points. Peak area was then recorded and recalculated to ng/ml according to calibration curve.
Skrott Z., Mistrik M., Andersen K.K., Friis S., Majera D., Gursky J., Ozdian T., Bartkova J., Turi Z., Moudry P., Kraus M., Michalova M., Vaclavkova J., Dzubak P., Vrobel I., Pouckova P., Sedlacek J., Miklovicova A., Kutt A., Li J., Mattova J., Driessen C., Dou Q.P., Olsen J., Hajduch M., Cvek B., Deshaies R.J, & Bartek J. (2017). Alcohol-abuse drug disulfiram targets cancer via p97 segregase adapter NPL4. Nature, 552(7684), 194-199.
+ Open protocol
+ Expand
2

Quantification of CuET in HPLC-ESI-QTOF

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HR-MRM analysis was performed on HPLC-ESI-QTOF system consisting of HPLC chromatograph Thermo UltiMate 3000 with AB Sciex TripleTOF 5600+ mass spectrometer, using the DuoSpray ESI source operated at ion source voltage 5500 V, ion source gas flow rates 40 units, curtain gas flow rate 30 units, declustering potential 100 V and temperature 400°C. Data were acquired in Product ion mode with two parent masses 358.9 and 360.9 for analysis of CuET. Chromatographic separation was done by PTFE column especially designed for analysis of strong metal chelators filled by C18 sorbent (IntellMed, cat.no.IM_301). Analysis was performed at room temperature and flow rate 1500 μL/min with isocratic chromatography. Mobile phase consisted of HPLC grade acetone (Lachner) 99.9%, HPLC water (Merck Millipore) 0.1% and 0.03% HPLC formic acid (Sigma). Acquired mass spectra were evaluated in software PeakView 1.2, where extracted ion chromatograms of transitions 88.0 and 116.0 (common for both parent masses) with 0.1 mass tolerance was Gaussian smoothened with width of 2 points. Peak area was then recorded and recalculated to ng/ml according to calibration curve.
Skrott Z., Mistrik M., Andersen K.K., Friis S., Majera D., Gursky J., Ozdian T., Bartkova J., Turi Z., Moudry P., Kraus M., Michalova M., Vaclavkova J., Dzubak P., Vrobel I., Pouckova P., Sedlacek J., Miklovicova A., Kutt A., Li J., Mattova J., Driessen C., Dou Q.P., Olsen J., Hajduch M., Cvek B., Deshaies R.J, & Bartek J. (2017). Alcohol-abuse drug disulfiram targets cancer via p97 segregase adapter NPL4. Nature, 552(7684), 194-199.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!