Mouse monoclonal antibodies against JEV NS3 and NS5 were generated in our laboratory. Commercially obtained antibodies used were: rabbit polyclonal anti-EEF1A1 (ABclonal Technology), mouse monoclonal anti-Flag (ABclonal Technology), mouse monoclonal anti-Myc (Abcam), mouse monoclonal anti-GAPDH (ABclonal Technology), mouse monoclonal anti-dsRNA J2 (English & Scientific Consulting Bt), horseradish peroxidase-conjugated anti-mouse and anti-rabbit IgG secondary antibodies (Boster), and Alexa Fluor 488-conjugated goat anti-mouse IgG and Alexa Fluor 555-conjugated goat anti-rabbit IgG (Invitrogen).
Mouse monoclonal anti gapdh
Manufactured by ABclonal
The Mouse monoclonal anti-GAPDH is a laboratory reagent used to detect and quantify the GAPDH protein in biological samples. GAPDH is a common housekeeping gene and its protein is widely used as a loading control in western blot and other protein analysis techniques.
Automatically generated - may contain errors
2 protocols using mouse monoclonal anti gapdh
1
Monoclonal Antibody Generation for JEV Proteins
2
Antibody Generation and Protein Detection Protocols
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Mouse monoclonal antibodies against JEV-NS5 protein, monoclonal antibodies against JEV E protein and FITC-labeled JEV-E antibodies were generated in our laboratory. Commercially obtained antibodies used were rabbit monoclonal anti-EZH2 (Cell Signaling Technology), rabbit monoclonal anti-H2K27me3 (ABclonal Technology), rabbit polyclonal anti-Histone H3 (ABclonal Technology), rabbit polyclonal anti-Rnf19a (ABclonal Technology), mouse monoclonal anti-Iba1 (Abacm), rabbit monoclonal anti-RIG-I (ABclonal Technology), mouse monoclonal anti-GAPDH (ABclonal Technology), rabbit monoclonal anti-ubiquitin (ABclonal Technology), rabbit monoclonal anti-HA-Tag (Proteintech), mouse monoclonal anti-DDDDK-Tag (ABclonal Technology), EZH2 inhibitor (GSK343, 10 μM, Selleck), lysosome inhibitor (NH4Cl, 10 mM, Sigma) and proteasome inhibitor (MG132, 10 μM, Sigma) in our experiments.
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