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3 protocols using disodium hydrogen phosphate

1

Irbesartan Formulation Development

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Irbesartan was procured from Yucca
Enterprises, Mumbai. Span 60,
cholesterol, disodium hydrogen phosphate, potassium dihydrogen phosphate,
ammonium acetate buffer, and mannitol were purchased from Loba Chemie,
Mumbai. Chloroform and acetonitrile were procured from Merck Pvt.,
Ltd. All solvents used were of HPLC grade, and the ingredients and
reagents used were of analytical grade. Distilled water was used for
all of the experiments.
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2

Evaluation of Quercetin and DCFDA Effects

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Quercetin hydrate (≥95%) and 2′,7′-dichlorodihydrofluoresceindiacetate (DCFDA, ≥97%) were procured from Merck, India. Roswell Park Memorial Institute (RPMI-1640) medium, Dulbecco’s Modified Eagle Medium (DMEM), antibiotics, fetal bovine serum, hematoxylin, and eosin were purchased from Himedia Ltd., India. All other reagents, including Sodium chloride, Potassium chloride, Disodium hydrogen phosphate, and Potassium dihydrogen phosphate, were purchased from Loba Chemie, India, and were of analytical grade.
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3

Optimization of Luteolin-loaded Formulation

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Luteolin was purchased from Beijing Mesochem Technology Co. Pvt. Ltd. (Beijing, China). Phospholipid 90G was received as a gift sample from Lipoid GmbH, Ludwigshafen, Germany. Sodium cholate, methyl paraben, triethanolamine and tween 80 were purchased from Sigma Aldrich, St Louis, MO, USA and Loba Chemie, Mumbai, India. Optimization of the formulation was performed using Design Expert software (Stat-Ease, Minneapolis, MN, USA). The cells were procured from the National Centre for Cell Science, Pune, India. Carbopol 934 and disodium hydrogen phosphate were procured from Loba Chemie Pvt. Ltd. Mumbai, India and Planet Science, Vadodra, India. The solvents methanol, chloroform, ethanol and water were used at analytical grade.
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