Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti actin

Manufactured by Fortis Life Sciences
Visit Supplier
Sourced in United States

Rabbit anti-Actin is a primary antibody that binds to actin, a cytoskeletal protein found in eukaryotic cells. It can be used in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and visualize actin in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti egfp, by Takara Bio (1 mentions) Odyssey m, by LI COR (1 mentions) Bis tris polyacrylamide gel, by Thermo Fisher Scientific (1 mentions) 6 well tissue culture treated plates, by Greiner (1 mentions) Cycloheximide (chx), by Cayman Chemical (1 mentions) Rabbit anti beclin 1, by Cell Signaling Technology (1 mentions) Alexa fluor 594 donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Mg 132, by InvivoGen (1 mentions) Rabbit anti p62, by Cell Signaling Technology (1 mentions) Mouse anti ha, by Merck Group (1 mentions) Rabbit anti lc3, by Cell Signaling Technology (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Goat anti rabbit igg, by LI COR (1 mentions) Odyssey scanner and software, by LI COR (1 mentions) Intercept blocking buffer, by LI COR (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Anti mouse irdye 680rd, by LI COR (1 mentions) Licor odyssey m, by LI COR (1 mentions) Anti rabbit irdye 800cw, by LI COR (1 mentions) Mouse monoclonal anti gfp b 2, by Santa Cruz Biotechnology (1 mentions)

5 protocols using rabbit anti actin

1

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
J-Lat 10.6 or 5A8 cells were plated in 6-well tissue culture-treated plates (Greiner Bio-one) at (5×106 cells/mL) in plain or drug media for 24 hrs. Cells were then lysed in radioimmunoprecipitation assay (RIPA) buffer (150 mM NaCl, 1% NP-40, 0.5% DOC, 0.1% SDS, 50 mM Tris) for 15 min on ice and clarified by centrifugation at 14,800 x g for 15 minutes. Lysates were boiled in 4x sample buffer (250 mM Tris-HCL, 8% SDS, 0.2% Bromophenol Blue, 40% Glycerol, 20% β-mercaptoethanol) in preparation for SDS-PAGE using 12% Bis-Tris polyacrylamide gels (Invitrogen) and transferred to polyvinylidene difluoride membranes. Immunoblotting was performed using mouse anti-eGFP (Takara, 1:2000), rabbit anti-Actin (Bethyl, 1:6000), mouse anti-gag (NIH-ARP#3537, 1:1000) followed by donkey anti-rabbit IgG 800CW (IRDye®,1:20000) or donkey anti-mouse IgG 680RD (IRDye®,1:20000) and imaged on a LI-COR Odyssey M.
Nichols Doyle R., Yang V., Damoiseaux R, & Fregoso O.I. (2023). NSC95397 is a Novel HIV Latency Reversing Agent. bioRxiv.
+ Open protocol
+ Expand
2

Autophagy and ISG15 Induction Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cycloheximide (catalog no. 14126) was purchased from Cayman Chemical (MI, USA), and MG-132 (catalog no. tlrl-mg132) was obtained from InvivoGen (CA, USA). Rabbit anti-LC3 (catalog no. 4108S), rabbit anti-Beclin1 (catalog no. 3738S), and rabbit anti-p62 (catalog no. 8025S) were purchased from Cell Signaling Technologies (MA, USA). Rabbit anti-actin (catalog no. A300-485A) was purchased from Bethyl Laboratories (TX, USA). Mouse anti-Flag (catalog no. F1804) and mouse anti-HA (catalog no. 26183) antibodies were obtained from Sigma-Aldrich (MO, USA) and ThermoFisher Scientific (MA, USA), respectively. Fluorescein isothiocyanate (FITC) goat anti-rabbit IgG (catalog no. 111-095-003) and Alexa Fluor 594 donkey anti-mouse IgG were purchased from Jackson ImmunoResearch Laboratories (PA, USA). Anti-ISG15 antibody was generated as described previously (46 (link)). Interferon beta (catalog no. 10704-HNAS) was purchased from Sino Biological US, Inc. (PA, USA).
Chiok K., Pokharel S.M., Mohanty I., Miller L.G., Gao S.J., Haas A.L., Tran K.C., Teng M.N, & Bose S. (2022). Human Respiratory Syncytial Virus NS2 Protein Induces Autophagy by Modulating Beclin1 Protein Stabilization and ISGylation. mBio, 13(1), e03528-21.
+ Open protocol
+ Expand
3

Western Blot Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer, and the following primary and secondary antibodies were used: rabbit anti-PABPC1 (Abcam), rabbit anti-actin (Bethyl), and goat-anti-rabbit IgG (Licor). Blots were visualized and scanned with Odyssey scanner and software (Li-Cor Bioscience).
Kini H.K., Silverman I.M., Ji X., Gregory B.D, & Liebhaber S.A. (2016). Cytoplasmic poly(A) binding protein-1 binds to genomically encoded sequences within mammalian mRNAs. RNA, 22(1), 61-74.
+ Open protocol
+ Expand
4

Western Blot Analysis of Cellular Proteins

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was collected from cells as previously described (28 (link)). Membranes were blocked in intercept blocking buffer (Li-COR Biosciences). Immunoblotting was performed using mouse anti-FLAG M2 (Sigma-Aldrich), rabbit anti-Actin (Bethyl), or mouse anti-DCAF1 (Proteintech) for 1hr. Blots were incubated with secondary antibodies IRDye 800CW anti-Rabbit and IRDye 680RD anti-Mouse (Li-COR Biosciences) for 1 hr and then visualized using the Li-COR Odyssey M (Li-COR Biosciences).
Sandoval C, & Fregoso O.I. (2023). HIV-1 Vpr-induced DNA damage activates NF-κB independent of cell cycle arrest and CRL4ADCAF1 engagement. bioRxiv.
+ Open protocol
+ Expand
5

Immunoblotting for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
CpG oligodeoxynucleotide-1826 (CpG DNA) and biotin-CpG DNA were purchased from TIB Molbiol (Germany). R848 and LPS (Escherichia coli O111:B4) were from Enzo Life Sciences (USA) and Sigma-Aldrich (USA), respectively. Rat monoclonal anti-HA (3F10) was purchased from Roche (USA). Mouse monoclonal anti-GFP (B-2) was purchased from Santa Cruz (USA). Mouse monoclonal anti-myc (9B11), rabbit monoclonal anti-MyD88 (D80F5), mouse monoclonal anti-phospho-Iκβ (5A5), and anti-Iκβ were obtained from Cell Signaling Technology (USA). Rat anti-LAMP1 (1D4B) was obtained from BD Biosciences (USA). Rabbit anti-actin was obtained from Bethyl Laboratories (USA). Rabbit anti-GFP and rabbit anti-myc antibodies were made in our laboratory by immunizing rabbits with purified GFP protein and Myc epitope tag peptide (EQKLISEEDL), respectively.
Song H.S., Park S., Huh J.W., Lee Y.R., Jung D.J., Yang C., Kim S.H., Kim H.M, & Kim Y.M. (2022). N-glycosylation of UNC93B1 at a Specific Asparagine Residue Is Required for TLR9 Signaling. Frontiers in Immunology, 13, 875083.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!