Uplc esi ms

Manufactured by Waters Corporation

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The UPLC/ESI-MS is an analytical instrument that combines Ultra-Performance Liquid Chromatography (UPLC) and Electrospray Ionization Mass Spectrometry (ESI-MS). It is designed to separate, identify, and quantify a wide range of chemical compounds with high resolution and sensitivity.

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Waters acquity uplc beh c18, by Waters Corporation (1 mentions) Aviii 600, by Bruker (1 mentions) Reverse phase hplc, by Waters Corporation (1 mentions) Hlb cartridge, by Waters Corporation (1 mentions) Beh c18 column, by Waters Corporation (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions)

3 protocols using uplc esi ms

Quantifying NOB Levels via UPLC/ESI-MS

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The measurement method for NOB was described in previous work [6 (link),7 (link)]. Briefly, the concentration of NOB was determined by ultra-performance liquid chromatography with electrospray ionization mass spectrometry (UPLC/ESI-MS) analysis (Waters, Milford, MA, USA). Waters Acquity UPLC BEH C18 (particle size: 1.7 μm, column size: 2.1 × 50 mm; Waters) was used. Samples were separated using a gradient mobile phase consisting of Milli-Q containing 5 mM ammonium acetate and acetonitrile, and analysis was carried out using selected ion recording (SIR), depending on mass-to-charge ratio of NOB and dexamethasone (internal standard).

Nihei T., Ushiro E., Sato H, & Onoue S. (2021). Biopharmaceutical Study on Nobiletin-Loaded Amorphous Solid Dispersion with Improved Hypouricemic Effect. Molecules, 26(15), 4447.

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Small Molecule Purification and Characterization

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The purification of small molecular compounds was performed by reverse-phase HPLC (Waters, USA) equipped with a Luna 5 µ C18 (2) 100 Å, 100 × 10.00 mm (Phenomenex, CA, USA) using MilliQ/0.1% TFA and acetonitrile/ 0.1% TFA. The gradient varied depending on the characteristics of the reaction product and the reaction crude. The measurement of the compounds was performed by UPLC-ESI-MS (Waters, USA) equipped with an ACQUITY UPLC BEH C18 1.7 µm 2.1 ×50 mm reverse-phase column (Waters, Milford, MA, USA) as stationary phase using a linear gradient from 100% MilliQ/0.1% formic acid to 100% acetonitrile/0.1% formic acid. The For ¹H NMR characterization, compounds were dissolved DMSO-d6 and the data were collected by Bruker AV III 600. The data are reported in terms of chemical shift (ppm), multiplicity, coupling constant, and signal integration.

Cui M., Nguyen D., Gaillez M.P., Heiden S., Lin W., Thompson M., Reddavide F.V., Chen Q, & Zhang Y. (2023). Trio-pharmacophore DNA-encoded chemical library for simultaneous selection of fragments and linkers. Nature Communications, 14, 1481.

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Quantitative Bile Acid Profiling

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The BA were measured by UPLC/ESI-MS (Waters) with 23-nor-5β-cholanic acid-3α,12α-diol (NDCA) as an internal standard according to our previous study (26) . The BA in the
freeze-dried samples were extracted with ethanol and then purified with an HLB cartridge (Waters) according to the manufacturer's instruction. The BA in each sample were separated using an Acquity UPLC system equipped with a BEH C 18 column (1•7 μM, 100 × 2•0 mm i.d.) (Waters) and were analysed on a Quattro Premier XE quadrupole tandem MS (Waters). The BA measured in this study were as follows: 5β-cholanic acid-3α,7α,12α-triol (CA); 5β-cholanic acid-3α,6β,7α-triol (α-muricholic acid; αMCA); 5β-cholanic acid-3α,6β,7β-triol (β-muricholic acid; βMCA); 5βcholanic acid-3α,6α,7β-triol (ω-muricholic acid; ωMCA); 5β-cholanic acid-3α,6α-diol (hyodeoxycholic acid; HDCA); 5β-cholanic acid-3α,7β-diol; 5β-cholanic acid-3α,7α-diol (CDCA); 5β-cholanic acid-3α,12α-diol (DCA); 5β-cholanic acid-3α-ol (LCA); 5β-cholanic acid-3α,6α,7α-triol; 5β-cholanic acid-3α,7β,12α-triol (ursocholic acid; UCA); 5β-cholanic acid-3α,12α-diol-7-one; 5β-cholanic acid-3α-ol-7-one; 5β-cholanic acid-3α-ol-12-one (12-oxolithocholic acid; 12oLCA); 5β-cholanic acid-12α-ol-3-one (3o12α

Lee Y., Yoshitsugu R., Kikuchi K., Joe G.H., Tsuji M., Nose T., Shimizu H., Hara H., Minamida K., Miwa K, & Ishizuka S. (2016). Combination of soya pulp and Bacillus coagulans lilac-01 improves intestinal bile acid metabolism without impairing the effects of prebiotics in rats fed a cholic acid-supplemented diet. The British journal of nutrition, 116(4).

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