Longissimus thoracis muscle biopsies were taken between the 7th and 9th ribs of 125 limousine bulls slaughtered at the age of 15.8 months. The samples were immediately frozen in liquid nitrogen and stored at -80°C. After grinding tissues using a FastPrep FP120 Homogenizer device (Thermo Savant) and micro-tubes “Lysing Matrix D” (MP Biomedicals), RNA extractions were performed with the RNeasy Midi/Maxi kit (Qiagen). The procedure and solution quantity were optimized for extraction from skeletal muscles and treatment with proteinase K as recommended by the supplier. The quality control of RNA step was done using RNA 6000 Nano Chips analyzed with 2100 Bioanalyzer instrument (Agilent Technologies). The 22 best ranking RNA samples were retained.
Rneasy midi maxi kit
Manufactured by Qiagen
Sourced in France
The RNeasy Midi/Maxi kit is a laboratory equipment product designed for the purification of total RNA from a variety of sample types. It utilizes a silica-membrane-based technology to efficiently capture and purify RNA molecules.
Automatically generated - may contain errors
2 protocols using rneasy midi maxi kit
1
Longissimus thoracis muscle RNA extraction
2
RNA Isolation from Salmonella-Infected HeLa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For RNA isolation, HeLa cells were infected with Salmonella strains for 10, 20 and 30 minutes, period in which effector proteins from T3SS1 were probably injected. Using three independent experiments, RNAs were isolated with the Qiagen RNeasy Midi/Maxi Kit (Qiagen, Courtaboeuf, France) and treated with DNase according to the manufacturer's instructions. cDNA samples and normalization of each gene to the 16S rRNA internal control were generated as previously described (Le Bars et al., 2012b) . Forward (F) and reverse (R)
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