Acquity xevo tqs

Fatty acids were detected via ultra-performance liquid chromatography (UPLC). Cell and plasma solutions were placed into 1.5 mL tubes, and 150 µL of cold CH₃CN was added for protein precipitation. Then, the solutions were vortexed and centrifuged at 15,000 × g for 10 min at 4 °C. The supernatants were transferred to clean tubes and evaporated. Sample analysis was carried out on an Acquity-Xevo TQS (Waters, Milford, USA) system.

The reactions were monitored by LC-DAD-MS, employing a liquid chromatograph coupled with a mass spectrometer Acquity Xevo TQS (Waters®), Ascentis® Express C18 column (10.0 cm × 3 mm, 2.7 μm) from Sigma-Aldrich. The setup included a DAD detector (200–700 nm) and both analysis modes, positive and negative, were employed to identify the reaction products.
For preparative High Performance Liquid Chromatography, a Shimadzu® HPLC system, equipped with CBM-20A controller, LC6AD bombs, SPD-20A detector, DGU-20A5 degas and Rheodyne manual injector, was used. Initially, a Shimadzu-ODS C18 column (250 × 4.6 mm, 5 μm) was used, followed by a Luna C18 column (250 × 20.00 mm, 5 μm) from Phenomenex®. The method was a linear gradient, mixture of methanol and water, both with 0.1% of formic acid, starting from the proportion 60 : 40 (MeOH/H₂O) to 98 (MeOH/H₂O) in 30 minutes. The flow rate was set at 1 mL min⁻¹ for the analytic mode and 12 mL min⁻¹ for preparative mode. The wavelength range used was 371–480 nm.