Human bronchial epithelial cells BEAS-2B cells (Sigma) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 U/ml) in 5% CO2 and humid air at 37°C. Human small airway epithelial cells SAECs (Lonza) were maintained in Small Airway Epithelial Cell Growth Medium (SAGM; basal medium plus growth supplements, Lonza). Cells were used between passage 4 and 10. Cells were exposed to CSE (0.25% for SAECs and 0.5% for BEAS-2B based on their viability) for 24 hours in the presence or absence of 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR, 1 mM, Sigma) and Compound C (5 μM, Sigma).
Saecs
Manufactured by Merck Group
Sourced in United States
SAECs are a type of lab equipment designed for use in scientific research and analysis. They are used to process and analyze small amounts of biological samples. The core function of SAECs is to provide precise and consistent results for various laboratory tests and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Compound c, by Merck Group (1 mentions)
Beas 2b, by Merck Group (1 mentions)
5 aminoimidazole 4 carboxamide ribonucleotide aicar, by Merck Group (1 mentions)
Beas 2b cells, by Merck Group (1 mentions)
Saecs, by Lonza (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Saecs, by American Type Culture Collection (1 mentions)
Poly 1 c, by Merck Group (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Bronchial epithelial cell growth kit, by American Type Culture Collection (1 mentions)
Il 13, by R&D Systems (1 mentions)
2 protocols using saecs
1
Cigarette Smoke Exposure Effects on Airway Cells
2
Alveolar Epithelial Cell Responses to Inflammatory Stimuli
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Two human AECs (type II alveolar epithelial cell line [A549] and primary small airway epithelial cells [SAECs]) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). A549 cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), and 1% penicillin-streptomycin (Gibco). SAECs were cultured in AEC basal medium (ATCC) plus bronchial epithelial cell growth kit (ATCC), 1% penicillin-streptomycin, and 25 ng/mL amphotericin B (Sigma-Aldrich, St. Louis, MO, USA).
SAECs (1 × 105) or A549 cells (2 × 105) were stimulated with poly I:C (1 or 10 µg/mL; Sigma-Aldrich), IL-13 (10 or 100 ng/mL; R&D Systems, Minneapolis, MN, USA), or phorbol myristate acetate (PMA)-induced EETs (100 ng/mL) for 24 hours or cocultured with 5 × 105 peripheral blood eosinophils (PBEs) from asthmatics for 18 hours. AECs were pretreated with 1 or 10 µg/mL dexamethasone (Dex; Sigma-Aldrich) for 30 minutes to evaluate its suppressive effect. Supernatants were collected for ELISA.
SAECs (1 × 105) or A549 cells (2 × 105) were stimulated with poly I:C (1 or 10 µg/mL; Sigma-Aldrich), IL-13 (10 or 100 ng/mL; R&D Systems, Minneapolis, MN, USA), or phorbol myristate acetate (PMA)-induced EETs (100 ng/mL) for 24 hours or cocultured with 5 × 105 peripheral blood eosinophils (PBEs) from asthmatics for 18 hours. AECs were pretreated with 1 or 10 µg/mL dexamethasone (Dex; Sigma-Aldrich) for 30 minutes to evaluate its suppressive effect. Supernatants were collected for ELISA.
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